Okazaki pieces grow opposite to the replication fork direction during simian virus 40 DNA replication.

Nucleic acids research ·Vol. 5 ·No. 7 ·1978-07-00 ·Pages 2535-45

Kaufmann G, Bar-Shavit R, DePamphilis ML

Abstract

Simian virus 40 replicating DNA was pulse labeled with alpha-32P-dATP using an acellular DNA replication system. Nascent DNA chains of less than 200 nucleotides (Okazaki pieces) were then isolated from the denatured replicating DNA by electrosieving through a polyacrylamide gel column. The purified Okazaki pieces were hybridized to separated strands of Bg1(1)+Hpa1 simian virus 40 DNA restriction fragments immobilized on nitrocellulose filters. Only strands with polarity of the DNA replication fork direction hybridized with Okazaki pieces. Hence, Okazaki pieces in simian virus 40 are synthesized against the DNA replication fork direction.

MeSH Terms

DNA Replication DNA-Directed DNA Polymerase/metabolism Molecular Weight Nucleic Acid Hybridization RNA, Viral/metabolism Simian virus 40/genetics Virus Replication

Chemicals

RNA, Viral DNA-Directed DNA Polymerase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kaufmann G

Bar-Shavit R

DePamphilis M L

References (15)

15 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/5.7.2535

Published

1978-07-00

Pages

2535-45

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342183

Subset

External Links

PubMed source DOI: 10.1093/nar/5.7.2535 PMC Full Text View journal details

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Okazaki pieces grow opposite to the replication fork direction during simian virus 40 DNA replication.

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