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PMID: 2102848 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Yeast RAS2 affects cell viability, mitotic division and transient gene expression in Nicotiana species.

Plant molecular biology ·Vol. 14 ·No. 5 ·1990-05-00 ·Pages 669-85

Hilson P, Dewulf J, Delporte F, Installé P, Jacquemin JM, Jacobs M, Negrutiu I

Abstract

Overexpression of the budding yeast RAS2 gene in Nicotiana plumbaginifolia cells revealed that RAS2 acted as 'suicide' gene in freshly isolated protoplasts from leaves and blocked cell proliferation in cell suspension-derived protoplasts. Among a series of genes tested (such as npt II, CDC35, PDE2), RAS2 was the only one to block the expression of the cat gene, as measured in a transient gene expression assay. Another ras gene, v-Ha-ras, had similar effects. Furthermore, the RAS2 effect was species-specific and depended on the modulation of hormonal metabolism in the transfected cells, while no differences were noticed between the normal and the activated val19 gene. Transfected plant cells are shown to synthesize a RAS2 protein of the same electrophoretic mobility as the yeast RAS2 product. The results are discussed in the broader context of the evolutionarily conserved ras genes involved in vital cellular functions.

MeSH Terms
Cell Differentiation/genetics Cell Survival/genetics Fungal Proteins/biosynthesis,genetics Gene Expression Genes, Fungal Genes, ras Genetic Engineering Mitosis/genetics Plant Cells Plants/genetics,metabolism Plants, Toxic Plasmids Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Tobacco/cytology,genetics Transformation, Genetic ras Proteins
Chemicals
Fungal Proteins Saccharomyces cerevisiae Proteins RAS2 protein, S cerevisiae ras Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Hilson P
Unité de Biologie Moléculaire et de Physiologie Animale, Faculté des Sciences Agronomiques de l'Etat, Gembloux, Belgium.
Dewulf J
Delporte F
Installé P
Jacquemin J M
Jacobs M
Negrutiu I
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00016500
Published
1990-05-00
Pages
669-85
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00016500 View journal details
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