Transient expression of chimaeric genes in dividing and non-dividing cereal protoplasts after PEG-induced DNA uptake.

Plant cell reports ·Vol. 6 ·No. 5 ·1987-10-00 ·Pages 329-32

Junker B, Zimny J, Lührs R, Lörz H

Abstract

Transient expression of chimaeric genes (neomycin phosphotransferase fused to four different promoters) was detected in suspension culture derived protoplasts of maize, barley and rice, in mesophyll protoplasts of maize, rice, rye, and root protoplasts of maize. The introduction and expression of foreign genes could be performed with both dividing and non-dividing protoplasts by applying the PEG transformation method. The significance of this method for the functional analysis of genes was demonstrated by the differential expression of a regulated gene in protoplasts of different tissues in agreement with its expression in the donor tissue.

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Junker B

Max-Planck-Institut für Züchtungsforschung, Am Engelspfad, D-5000, Köln 30, Federal Republic of Germany.

Zimny J

Lührs R

Lörz H

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13 references, click to expand

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Article Info

Journal

Plant cell reports

Abbr.

Plant Cell Rep

ISSN

0721-7714

DOI

10.1007/BF00269552

Published

1987-10-00

Pages

329-32

Language

English

Region

Germany

NLM ID

9880970

External Links

PubMed source DOI: 10.1007/BF00269552 View journal details

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Transient expression of chimaeric genes in dividing and non-dividing cereal protoplasts after PEG-induced DNA uptake.

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