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PMID: 3862099 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression of genes transferred into monocot and dicot plant cells by electroporation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 17 ·1985-09-00 ·Pages 5824-8

Fromm M, Taylor LP, Walbot V

Abstract

We have developed a general method for electrically introducing DNA into plant cells. Gene transfer occurs when a high-voltage electric pulse is applied to a solution containing protoplasts and DNA. Carrot protoplasts were used as a model system to optimize gene-transfer efficiency, which was measured 24-48 hr after electroporation by the amount of chloramphenicol acetyltransferase activity resulting from the expression of the introduced chimeric plasmids. Gene-transfer efficiency increased with the DNA concentration and was affected by the amplitude and duration of the electric pulse as well as by the composition of the electroporation medium. Our optimized gene-transfer conditions were effective when applied to tobacco and maize protoplasts, demonstrating that the method is applicable to both monocot and dicot protoplasts.

MeSH Terms
Acetyltransferases/genetics Cell Membrane Permeability Chloramphenicol O-Acetyltransferase Electricity Gene Expression Regulation Genetic Engineering/methods Plants/genetics Plasmids Transformation, Genetic
Chemicals
Acetyltransferases Chloramphenicol O-Acetyltransferase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fromm M
Taylor L P
Walbot V
References (20)
20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.17.5824
Published
1985-09-00
Pages
5824-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390645
Subset
IM
Grants
NCI NIH HHS · CA09302 · United States
NIGMS NIH HHS · GM32422 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.17.5824 PMC Full Text View journal details
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