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PMID: 2103464 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Length heterogeneity of the rRNA precursor in cucumber (Cucumis sativus).

Plant molecular biology ·Vol. 15 ·No. 3 ·1990-09-00 ·Pages 465-74

Zentgraf U, Ganal M, Hemleben V

Abstract

The length homogeneous part of the intergenic spacer (IGS) of the 18S-25S ribosomal RNA genes of cucumber (Cucumis sativus) was characterized by sequencing 2389 bp preceding the 18S rRNA coding region of a 12.5 kbp repeat type. This part of the IGS is composed of repeated elements and shows a very complex structural organization. Most obvious is a 119 bp element which is repeated seven times. A single transcription initiation site (TIS) was detected by a 'T4 polymerase stop' experiment upstream of these repetitions giving rise to a 2013 bp 5' external transcribed spacer (ETS) for cucumber. Nuclease mapping showed several transcription termination sites (TTS): the first one is located 350 bp downstream of the 25S rRNA coding region, the others are found within the duplications of this region accounting for the length heterogeneity of cucumber rDNA. Therefore, the TTS is repeated two or three times in the IGS depending on the length of the respective repeat classes and the rRNA precursor is heterogeneous in length varying from approx. 8000 to 11000 nts.

MeSH Terms
Base Sequence DNA, Ribosomal/genetics Genes, Plant Molecular Sequence Data Plants/genetics RNA Precursors/genetics RNA, Ribosomal, 18S/genetics Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid Species Specificity Terminator Regions, Genetic
Chemicals
DNA, Ribosomal RNA Precursors RNA, Ribosomal, 18S
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zentgraf U
Biologisches Institut der Universität Tübingen, FRG.
Ganal M
Hemleben V
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00019163
Published
1990-09-00
Pages
465-74
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
X51542
External Links
PubMed source DOI: 10.1007/BF00019163 View journal details
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