Home LiteratureArticle Details
PMID: 3725589 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The structure of the maize ribosomal DNA spacer region.

Nucleic acids research ·Vol. 14 ·No. 12 ·1986-06-25 ·Pages 4953-68

McMullen MD, Hunter B, Phillips RL, Rubenstein I

Abstract

A combination of a single stranded plasmid vector and ordered deletions were used to determine the complete nucleotide sequence of a rDNA spacer region isolated from the DNA of maize Black Mexican Sweet suspension cells. The sequence reveals the presence of ten "200 base" subrepeats within the maize rDNA spacer region. By S1 protection experiments we have tentatively determined that the start of maize rRNA transcription is 144 bases 3' of the end of the last spacer subrepeat. We propose that the spacer subrepeats may have an important role in regulating maize rRNA transcription.

MeSH Terms
Base Sequence Chromosome Mapping DNA, Ribosomal/genetics Enhancer Elements, Genetic Gene Expression Regulation RNA, Ribosomal/genetics Transcription, Genetic Zea mays/genetics
Chemicals
DNA, Ribosomal RNA, Ribosomal
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
McMullen M D
Hunter B
Phillips R L
Rubenstein I
References (32)
32 references, click to expand
  1. A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn mitochondrial 18 S rDNA.
    Plasmid. 1985 Jan;13(1):31-40 PMID: 3991809
  2. Spacer regulation of Xenopus ribosomal gene transcription: competition in oocytes.
    Cell. 1983 Dec;35(2 Pt 1):449-56 PMID: 6652676
  3. Multiple Pol I initiation sequences in rDNA spacers of Drosophila melanogaster.
    Nucleic Acids Res. 1982 Nov 11;10(21):7017-26 PMID: 6294625
  4. Elution of DNA from agarose gels after electrophoresis.
    Methods Enzymol. 1979;68:176-82 PMID: 232211
  5. Characterisation of the genes for ribosomal RNA in flax.
    Nucleic Acids Res. 1981 Mar 25;9(6):1301-9 PMID: 6262724
  6. Transcription of the 'non-transcribed' spacer of Drosophila melanogaster rDNA.
    Nucleic Acids Res. 1983 Jan 11;11(1):11-9 PMID: 6306560
  7. Nontranscribed spacer sequences promote in vitro transcription of Drosophila ribosomal DNA.
    Nucleic Acids Res. 1982 Nov 11;10(21):6879-86 PMID: 6817304
  8. DNase I footprinting shows three protected regions in the promoter of the rRNA genes of Xenopus laevis.
    Mol Cell Biol. 1985 Feb;5(2):313-9 PMID: 4038778
  9. Transposition in plants: a molecular model.
    EMBO J. 1985 Mar;4(3):585-90 PMID: 15926218
  10. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  11. Processing of RNA.
    Annu Rev Biochem. 1976;45:605-29 PMID: 786158
  12. Restriction analysis of spacers in ribosomal DNA of Drosophila melanogaster.
    Nucleic Acids Res. 1979 Sep 11;7(1):205-15 PMID: 114986
  13. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  14. Amplified ribosomal DNA from Xenopus laevis has heterogeneous spacer lengths.
    Proc Natl Acad Sci U S A. 1974 Jul;71(7):2823-7 PMID: 4527647
  15. Cloning and sequencing of the ribosomal RNA genes in maize: the 17S region.
    DNA. 1984;3(1):31-40 PMID: 6365492
  16. Visualization of nucleolar genes.
    Science. 1969 May 23;164(3882):955-7 PMID: 5813982
  17. A transcriptional function for the repetitive ribosomal spacer in Xenopus laevis.
    Nature. 1983 Mar 17-23;302(5905):223-8 PMID: 6835360
  18. Heterogeneity of pumpkin ribosomal DNA.
    Plant Physiol. 1983 May;72(1):166-71 PMID: 16662953
  19. Nucleotide sequence of a complete ribosomal spacer of D. melanogaster.
    Nucleic Acids Res. 1985 Feb 25;13(4):1089-101 PMID: 3923435
  20. Enhancer-like properties of the 60/81 bp elements in the ribosomal gene spacer of Xenopus laevis.
    Cell. 1984 May;37(1):285-9 PMID: 6722873
  21. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  22. Expression of genes transferred into monocot and dicot plant cells by electroporation.
    Proc Natl Acad Sci U S A. 1985 Sep;82(17):5824-8 PMID: 3862099
  23. The organization of a nuclear DNA sequence from a higher plant: molecular cloning and characterization of soybean ribosomal DNA.
    Gene. 1979 Nov;7(3-4):317-34 PMID: 160361
  24. Sequence organization of the spacer DNA in a ribosomal gene unit of Xenopus laevis.
    Cell. 1979 May;17(1):19-31 PMID: 455459
  25. The transcription termination site of the ribosomal RNA operon in yeast.
    Nucleic Acids Res. 1980 Nov 25;8(22):5179-92 PMID: 6258138
  26. Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics.
    Proc Natl Acad Sci U S A. 1984 Dec;81(24):8014-8 PMID: 6096873
  27. Mapping of transcription initiation and termination signals on Xenopus laevis ribosomal DNA.
    Proc Natl Acad Sci U S A. 1982 Jan;79(1):56-60 PMID: 6948303
  28. The organization and transcription of eukaryotic ribosomal RNA genes.
    Prog Nucleic Acid Res Mol Biol. 1984;31:115-60 PMID: 6397769
  29. The nucleotide sequence of the initiation and termination sites for ribosomal RNA transcription in X. laevis.
    Cell. 1979 Oct;18(2):485-99 PMID: 498280
  30. In vivo transcription of rDNA spacers in Drosophila.
    Nucleic Acids Res. 1985 May 10;13(9):3221-39 PMID: 2987877
  31. Repeated genes in eukaryotes.
    Annu Rev Biochem. 1980;49:727-64 PMID: 6996571
  32. The nucleotide sequence at the transcription termination site of ribosomal RNA in Drosophila melanogaster.
    Nucleic Acids Res. 1981 Apr 24;9(8):1801-11 PMID: 6264393
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.12.4953
Published
1986-06-25
Pages
4953-68
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311503
Subset
IM
Databases
GENBANK
X03990
External Links
PubMed source DOI: 10.1093/nar/14.12.4953 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2