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PMID: 6264393 Published · ppublish English Journal Article

The nucleotide sequence at the transcription termination site of ribosomal RNA in Drosophila melanogaster.

Nucleic acids research ·Vol. 9 ·No. 8 ·1981-04-24 ·Pages 1801-11

Mandal RK, Dawid IB

Abstract

The sequence of 300 nucleotides surrounding the transcription termination site for ribosomal RNA in Drosophila melanogaster has been determined. The precise position of the RNA 3'-end was located by sizing rDNA fragments protected from S1 nuclease by hybridization with cytoplasmic 28S rRNA or with nuclear 38S pre-rRNA. Next, the sequence of the first 10 nucleotides at the 3'-end of 28S and 38S rRNA was determined directly. The results indicate the absence of 3'-terminal processing of the 38S pre-rRNA. The nucleotide sequence from a position 27 nucleotides upstream to 150 nucleotides downstream from the termination site was identical in three uninterrupted rRNA genes and one interrupted gene, except for a single base change in the spacer region of one clone. The terminal 100 nucleotides of Drosophila 28S rRNA have more than 60 per cent homology with the corresponding region of Xenopus 28S rRNA and yeast 26S rRNA.

MeSH Terms
Animals Base Sequence DNA/genetics DNA Restriction Enzymes Drosophila melanogaster/genetics Molecular Weight RNA, Ribosomal/genetics Transcription, Genetic
Chemicals
RNA, Ribosomal DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mandal R K
Dawid I B
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24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.8.1801
Published
1981-04-24
Pages
1801-11
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326804
Subset
IM
Databases
GENBANK
K00466, K00467, K00468, V00232
External Links
PubMed source DOI: 10.1093/nar/9.8.1801 PMC Full Text View journal details
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