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PMID: 6162156 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The nucleotide sequence of the putative transcription initiation site of a cloned ribosomal RNA gene of the mouse.

Nucleic acids research ·Vol. 8 ·No. 24 ·1980-12-20 ·Pages 6043-58

Urano Y, Kominami R, Mishima Y, Muramatsu M

Abstract

Approximately one kilobase pairs surrounding and upstream the transcription initiation site of a cloned ribosomal DNA (rDNA) of the mouse were sequenced. The putative transcription initiation site was determined by two independent methods: one nuclease S1 protection and the other reverse transcriptase elongation mapping using isolated 45S ribosomal RNA precursor (45S RNA) and appropriate restriction fragments of rDNA. Both methods gave an identical result; 45S RNA had a structure starting from ACTCTTAG---. Characteristically, mouse rDNA had many T clusters (greater than or equal to 5) upstream the initiation site, the longest being 21 consecutive T's. A pentadecanucleotide, TGCCTCCCGAGTGCA, appeared twice within 260 nucleotides upstream the putative initiation site. No such characteristic sequences were found downstream this site. Little similarity was found in the upstream of the transcription initiation site between the mouse, Xenopus laevis and Saccharomyces cerevisiae rDNA.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant Electrophoresis, Polyacrylamide Gel Endonucleases Mice Nucleic Acid Hybridization RNA, Ribosomal/genetics RNA-Directed DNA Polymerase Saccharomyces cerevisiae/genetics Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic Xenopus laevis/genetics
Chemicals
DNA, Recombinant RNA, Ribosomal RNA-Directed DNA Polymerase Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Urano Y
Kominami R
Mishima Y
Muramatsu M
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42 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.24.6043
Published
1980-12-20
Pages
6043-58
Language
English
Region
England
NLM ID
0411011
PMCID
PMC328071
Subset
IM
Databases
GENBANK
J00622
External Links
PubMed source DOI: 10.1093/nar/8.24.6043 PMC Full Text View journal details
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