Home LiteratureArticle Details
PMID: 3923435 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of a complete ribosomal spacer of D. melanogaster.

Nucleic acids research ·Vol. 13 ·No. 4 ·1985-02-25 ·Pages 1089-101

Simeone A, La Volpe A, Boncinelli E

Abstract

We determined the nucleotide sequence of a D. melanogaster ribosomal DNA spacer. Sequences of various portions of different cloned ribosomal spacers have been previously reported. We extend the analysis to cover the entire nontranscribed and external transcribed regions. Comparison to other cloned ribosomal DNA gene units of this species confirms a conserved general organization of the ribosomal spacer through different size classes. D. melanogaster ribosomal gene units interrupted by insertions are known to be transcribed at a much lower level than the continuous gene units. Nonetheless previous sequence analysis of a region around the transcription initiation site did not reveal significant differences in rDNA genes with and without insertions. We extend such analysis to cover the last two promoter duplications in the spacer and the entire external transcribed spacer up to the 5' cleavage site of the 18S rRNA.

MeSH Terms
Animals Base Sequence Cloning, Molecular Drosophila melanogaster/genetics Genetic Linkage Nucleic Acid Precursors/genetics RNA Processing, Post-Transcriptional RNA, Ribosomal/genetics Transcription, Genetic
Chemicals
Nucleic Acid Precursors RNA, Ribosomal
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Simeone A
La Volpe A
Boncinelli E
References (43)
43 references, click to expand
  1. Nucleotide sequence of the rightward operator of phage lambda.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 PMID: 1055375
  2. The rare transcripts of interrupted rRNA genes in Drosophila melanogaster are processed or degraded during synthesis.
    EMBO J. 1984 Jul;3(7):1541-5 PMID: 6430695
  3. Processing of RNA.
    Annu Rev Biochem. 1976;45:605-29 PMID: 786158
  4. The molecular basis for length heterogeneity in ribosomal DNA from Xenopus laevis.
    J Mol Biol. 1976 Aug 25;105(4):461-86 PMID: 787539
  5. A simple method for DNA restriction site mapping.
    Nucleic Acids Res. 1976 Sep;3(9):2387-98 PMID: 787937
  6. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  7. X and Y chromosomal ribosomal DNA of Drosophila: comparison of spacers and insertions.
    Cell. 1978 Jun;14(2):269-78 PMID: 96940
  8. Length heterogeneity in a region of the human ribosomal gene spacer is not accompanied by extensive population polymorphism.
    J Mol Biol. 1978 Nov 25;126(1):91-104 PMID: 739545
  9. Ribosomal DNA in Drosophila melanogaster. I. Isolation and characterization of cloned fragments.
    J Mol Biol. 1978 Dec 25;126(4):749-68 PMID: 106128
  10. Ribosomal DNA in Drosophila melanogaster. II. Heteroduplex mapping of cloned and uncloned rDNA.
    J Mol Biol. 1978 Dec 25;126(4):769-82 PMID: 106129
  11. On spacers.
    Cell. 1979 Apr;16(4):697-710 PMID: 378397
  12. Sequence organization of the spacer DNA in a ribosomal gene unit of Xenopus laevis.
    Cell. 1979 May;17(1):19-31 PMID: 455459
  13. Restriction analysis of spacers in ribosomal DNA of Drosophila melanogaster.
    Nucleic Acids Res. 1979 Sep 11;7(1):205-15 PMID: 114986
  14. Expression of ribosomal DNA insertions in Drosophila melanogaster.
    Cell. 1979 Dec;18(4):1185-96 PMID: 117903
  15. Sequence and secondary structure of Drosophila melanogaster 5.8S and 2S rRNAs and of the processing site between them.
    Nucleic Acids Res. 1979 Dec 20;7(8):2213-38 PMID: 118436
  16. Nuclear RNA transcripts from Drosophila melanogaster ribosomal RNA genes containing introns.
    Nucleic Acids Res. 1980 Jan 11;8(1):67-84 PMID: 6243780
  17. Repeated genes in eukaryotes.
    Annu Rev Biochem. 1980;49:727-64 PMID: 6996571
  18. Sequence of the 3'-terminal portion of Drosophila melanogaster 18 S rRNA and of the adjoining spacer: comparison with corresponding prokaryotic and eukaryotic sequences.
    FEBS Lett. 1980 Aug 11;117(1):227-31 PMID: 6250894
  19. Alternative pathways in the processing of ribosomal RNA precursor in Drosophila melanogaster.
    J Mol Biol. 1980 Apr 25;138(4):873-8 PMID: 6774101
  20. More ribosomal spacer sequences from Xenopus laevis.
    Nucleic Acids Res. 1980 Feb 11;8(3):467-85 PMID: 7003549
  21. Nucleotide sequence of the initiation site for ribosomal RNA transcription in Drosophila melanogaster: comparison of genes with and without insertions.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1513-7 PMID: 6262809
  22. The nucleotide sequence at the transcription termination site of ribosomal RNA in Drosophila melanogaster.
    Nucleic Acids Res. 1981 Apr 24;9(8):1801-11 PMID: 6264393
  23. RNA polymerase I promoters and transcription factors.
    Nature. 1984 Jul 19-25;310(5974):189-90 PMID: 6431293
  24. Enhancers and ribosomal gene spacers.
    Cell. 1984 Sep;38(2):349-51 PMID: 6467370
  25. Expression of the ribosomal DNA insertions in bobbed mutants of Drosophila melanogaster.
    Mol Gen Genet. 1981;182(3):377-84 PMID: 6795426
  26. Sequence organization of the spacer in the ribosomal genes of Xenopus clivii and Xenopus borealis.
    Nucleic Acids Res. 1981 Oct 24;9(20):5311-30 PMID: 6272218
  27. Drosophila melanogaster ribosomal DNA containing type II insertions is variably transcribed in different strains and tissues.
    J Mol Biol. 1981 Oct 5;151(4):645-62 PMID: 6276565
  28. Accurate transcription of truncated ribosomal DNA templates in a Drosophila cell-free system.
    Proc Natl Acad Sci U S A. 1982 Mar;79(5):1501-5 PMID: 6803243
  29. Nucleotide sequence of an external transcribed spacer in Xenopus laevis rDNA: sequences flanking the 5' and 3' ends of 18S rRNA are non-complementary.
    Nucleic Acids Res. 1982 Apr 10;10(7):2387-98 PMID: 6283480
  30. Human ribosomal RNA gene: nucleotide sequence of the transcription initiation region and comparison of three mammalian genes.
    Proc Natl Acad Sci U S A. 1982 May;79(10):3092-6 PMID: 6954460
  31. Chemical modification of everninomicins.
    J Antibiot (Tokyo). 1982 May;35(5):561-70 PMID: 7107521
  32. Molecular drive: a cohesive mode of species evolution.
    Nature. 1982 Sep 9;299(5879):111-7 PMID: 7110332
  33. Nontranscribed spacer sequences promote in vitro transcription of Drosophila ribosomal DNA.
    Nucleic Acids Res. 1982 Nov 11;10(21):6879-86 PMID: 6817304
  34. Multiple Pol I initiation sequences in rDNA spacers of Drosophila melanogaster.
    Nucleic Acids Res. 1982 Nov 11;10(21):7017-26 PMID: 6294625
  35. Sequence organization of the ribosomal spacer of D.melanogaster.
    Nucleic Acids Res. 1982 Dec 20;10(24):8263-72 PMID: 6298714
  36. Structural organization of rat ribosomal genes restriction endonuclease analysis of genomic and cloned ribosomal DNAs.
    Gene. 1982 Dec;20(2):145-56 PMID: 6187625
  37. Nucleotide sequence of the transcriptional initiation region of Dictyostelium discoideum rRNA gene and comparison of the initiation regions of three lower eukaryotes' genes.
    Nucleic Acids Res. 1983 Mar 25;11(6):1725-34 PMID: 6300775
  38. Transcription of the 'non-transcribed' spacer of Drosophila melanogaster rDNA.
    Nucleic Acids Res. 1983 Jan 11;11(1):11-9 PMID: 6306560
  39. Secondary structure of the Tetrahymena ribosomal RNA intervening sequence: structural homology with fungal mitochondrial intervening sequences.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):3903-7 PMID: 6306649
  40. 5'-Cleavage site of D. melanogaster 18 S rRNA.
    FEBS Lett. 1984 Feb 27;167(2):249-53 PMID: 6421619
  41. Localization and DNA sequence around the initiation site of ribosomal RNA transcription in Physarum polycephalum.
    Nucleic Acids Res. 1983 Dec 10;11(23):8519-33 PMID: 6324087
  42. Structure and function of the nontranscribed spacer regions of yeast rDNA.
    Nucleic Acids Res. 1984 Mar 26;12(6):2955-68 PMID: 6369254
  43. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.4.1089
Published
1985-02-25
Pages
1089-101
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341058
Subset
IM
Databases
GENBANK
X02210, X02211
External Links
PubMed source DOI: 10.1093/nar/13.4.1089 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2