Home LiteratureArticle Details
PMID: 6298714 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequence organization of the ribosomal spacer of D.melanogaster.

Nucleic acids research ·Vol. 10 ·No. 24 ·1982-12-20 ·Pages 8263-72

Simeone A, de Falco A, Macino G, Boncinelli E

Abstract

We subcloned several fragments of the rDNA nontranscribed spacer (NTS) of D.melanogaster and analysed their nucleotide sequence. The central portion contains a variable number of 240 base pair repeated sequences ending with two contiguous Alu I sites. Within every repeat there is a 42 base pair sequence perfectly homologous to the transcription initiation site from -18 to +24. Sequences homologous to the basic 240 bp repeat are present both at the end of the unique 1900 bp region downstream to the transcription termination site and within the unique region preceding the transcription initiation site up to nucleotide -140. These results are discussed in relation to the general problem of ribosomal gene units and their evolution.

MeSH Terms
Animals Base Composition Cloning, Molecular DNA/genetics DNA Restriction Enzymes DNA, Ribosomal Drosophila melanogaster/genetics RNA, Ribosomal/genetics Repetitive Sequences, Nucleic Acid Transcription, Genetic
Chemicals
DNA, Ribosomal RNA, Ribosomal DNA DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Simeone A
de Falco A
Macino G
Boncinelli E
References (31)
31 references, click to expand
  1. Amplification as a rectification mechanism for the redundant rRNA genes.
    Nat New Biol. 1972 Aug 2;238(83):134-7 PMID: 4538238
  2. Identification of the in vivo and in vitro origin of transcription in human rDNA.
    Nucleic Acids Res. 1982 Jul 10;10(13):3933-49 PMID: 6287426
  3. Eukaryotic RNA polymerases and the factors that control them.
    Prog Nucleic Acid Res Mol Biol. 1975;15(0):145-84 PMID: 166413
  4. Lengths and patterns of transcriptional units in the amplified nucleoli of oocytes of Xenopus laevis.
    Chromosoma. 1977 Mar 16;60(2):147-67 PMID: 870292
  5. Circular ribosomal DNA during ribosomal magnification in Drosophila melanogaster.
    J Mol Biol. 1977 May 5;112(1):49-63 PMID: 407367
  6. Processing steps and methylation in the formation of the ribosomal RNA of cultured Drosophila cells.
    J Mol Biol. 1978 May 15;121(2):219-38 PMID: 660652
  7. Sequence organization of the spacer DNA in a ribosomal gene unit of Xenopus laevis.
    Cell. 1979 May;17(1):19-31 PMID: 455459
  8. Restriction analysis of spacers in ribosomal DNA of Drosophila melanogaster.
    Nucleic Acids Res. 1979 Sep 11;7(1):205-15 PMID: 114986
  9. Transcription of spacer sequences in genes coding for ribosomal RNA in Xenopus cells.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3957-61 PMID: 291055
  10. The nucleotide sequence of the initiation and termination sites for ribosomal RNA transcription in X. laevis.
    Cell. 1979 Oct;18(2):485-99 PMID: 498280
  11. Expression of ribosomal DNA insertions in Drosophila melanogaster.
    Cell. 1979 Dec;18(4):1185-96 PMID: 117903
  12. Sequence and secondary structure of Drosophila melanogaster 5.8S and 2S rRNAs and of the processing site between them.
    Nucleic Acids Res. 1979 Dec 20;7(8):2213-38 PMID: 118436
  13. Nuclear RNA transcripts from Drosophila melanogaster ribosomal RNA genes containing introns.
    Nucleic Acids Res. 1980 Jan 11;8(1):67-84 PMID: 6243780
  14. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  15. Repeated genes in eukaryotes.
    Annu Rev Biochem. 1980;49:727-64 PMID: 6996571
  16. Alternative pathways in the processing of ribosomal RNA precursor in Drosophila melanogaster.
    J Mol Biol. 1980 Apr 25;138(4):873-8 PMID: 6774101
  17. Transposable elements.
    Cell. 1980 Jul;20(3):579-95 PMID: 6251969
  18. Characterization of cloned ribosomal DNA from Drosophila hydei.
    Nucleic Acids Res. 1980 Oct 24;8(20):4593-611 PMID: 6255425
  19. The nucleotide sequence of the putative transcription initiation site of a cloned ribosomal RNA gene of the mouse.
    Nucleic Acids Res. 1980 Dec 20;8(24):6043-58 PMID: 6162156
  20. Evolution of the 87A and 87C heat-shock loci in Drosophila.
    Nature. 1981 Apr 23;290(5808):677-82 PMID: 6261150
  21. Orphons: dispersed genetic elements derived from tandem repetitive genes of eucaryotes.
    Cell. 1981 Mar;23(3):651-63 PMID: 6784929
  22. Nucleotide sequence of the initiation site for ribosomal RNA transcription in Drosophila melanogaster: comparison of genes with and without insertions.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1513-7 PMID: 6262809
  23. The nucleotide sequence at the transcription termination site of ribosomal RNA in Drosophila melanogaster.
    Nucleic Acids Res. 1981 Apr 24;9(8):1801-11 PMID: 6264393
  24. Nontranscribed spacers in Drosophila ribosomal DNA.
    Chromosoma. 1981;82(5):637-55 PMID: 6266780
  25. Sequence organization of the spacer in the ribosomal genes of Xenopus clivii and Xenopus borealis.
    Nucleic Acids Res. 1981 Oct 24;9(20):5311-30 PMID: 6272218
  26. Drosophila melanogaster ribosomal DNA containing type II insertions is variably transcribed in different strains and tissues.
    J Mol Biol. 1981 Oct 5;151(4):645-62 PMID: 6276565
  27. Rate of turnover of structural variants in the rDNA gene family of Drosophila melanogaster.
    Nature. 1982 Feb 18;295(5850):564-8 PMID: 6799840
  28. Ribosomal RNA transcription in vitro is species specific.
    Nature. 1982 Mar 11;296(5853):173-4 PMID: 7063022
  29. Accurate transcription of truncated ribosomal DNA templates in a Drosophila cell-free system.
    Proc Natl Acad Sci U S A. 1982 Mar;79(5):1501-5 PMID: 6803243
  30. Molecular drive: a cohesive mode of species evolution.
    Nature. 1982 Sep 9;299(5879):111-7 PMID: 7110332
  31. Procedure for magnification of lethal deletions of genes for ribosomal RNA.
    Nat New Biol. 1972 Nov 22;240(99):109-11 PMID: 4509021
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.24.8263
Published
1982-12-20
Pages
8263-72
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327083
Subset
IM
Databases
GENBANK
K01529, K01530, K01531, K01532, K01533
External Links
PubMed source DOI: 10.1093/nar/10.24.8263 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2