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PMID: 6300775 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of the transcriptional initiation region of Dictyostelium discoideum rRNA gene and comparison of the initiation regions of three lower eukaryotes' genes.

Nucleic acids research ·Vol. 11 ·No. 6 ·1983-03-25 ·Pages 1725-34

Hoshikawa Y, Iida Y, Iwabuchi M

Abstract

The 5' end of the rRNA precursor of D. discoideum was mapped on a cloned rDNA by S1 nuclease protection mapping, and the sequence of about 1240 nucleotides surrounding the transcriptional initiation site of the rRNA gene has been determined. Repeated sequences consisting of 16 nucleotides appeared in the region upstream from the initiation point. Comparison of the nucleotide sequences around the initiation site of rRNA genes in three lower eukaryotes, D. discoideum, Saccharomyces cerevisiae and Tetrahymena pyriformis, indicated that there was little similarity in the nontranscribed spacer regions, but in the transcribed spacer regions near the initiation point, very similar sequences consisting of 9 nucleotides were found.

MeSH Terms
Animals Base Sequence DNA/genetics DNA Restriction Enzymes DNA, Ribosomal Dictyostelium/genetics Genes RNA, Ribosomal/genetics Saccharomyces cerevisiae/genetics Species Specificity Tetrahymena pyriformis/genetics Transcription, Genetic
Chemicals
DNA, Ribosomal RNA, Ribosomal DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hoshikawa Y
Iida Y
Iwabuchi M
References (24)
24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.6.1725
Published
1983-03-25
Pages
1725-34
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325831
Subset
IM
Databases
GENBANK
V00189
External Links
PubMed source DOI: 10.1093/nar/11.6.1725 PMC Full Text View journal details
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