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PMID: 6278446 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

In vitro transcription of a cloned mouse ribosomal RNA gene.

Nucleic acids research ·Vol. 9 ·No. 24 ·1981-12-21 ·Pages 6773-85

Mishima Y, Yamamoto O, Kominami R, Muramatsu M

Abstract

An in vitro transcription system which utilizes cloned mouse ribosomal RNA gene (rDNA) fragments and a mouse cell extract has been developed. RNA polymerases I is apparently responsible for this transcription as evidenced by the complete resistance to a high concentration (200 micrograms/ml) of alpha-amanitin. Run-off products obtained with three different truncated rDNA fragments indicated that RNA was transcribed from a unique site of rDNA. The S1 nuclease protection mapping of the in vitro product and of in vivo 45S RNA confirmed this site, indicating that, in this in vitro system, transcription of rDNA started from the same site as in vivo. This site is located at several hundred nucleotides upstream from the putative initiation site reported by us (1) and by others (2). Some sequence homology surrounding this region was noted among mouse, Xenopus laevis and Drosophila melanogaster. The data also suggest that some processing of the primary transcript occurs in this in vitro system.

MeSH Terms
Animals Cloning, Molecular DNA, Recombinant Endonucleases Gene Expression Regulation Genes In Vitro Techniques Mice RNA, Ribosomal/genetics Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic
Chemicals
DNA, Recombinant RNA, Ribosomal Endonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Mishima Y
Yamamoto O
Kominami R
Muramatsu M
References (26)
26 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.24.6773
Published
1981-12-21
Pages
6773-85
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327640
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/9.24.6773 PMC Full Text View journal details
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