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PMID: 6262732 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The nucleotide sequence of the initiation region of the ribosomal transcription unit from mouse.

Nucleic acids research ·Vol. 9 ·No. 7 ·1981-04-10 ·Pages 1559-69

Bach R, Grummt I, Allet B

Abstract

The 5' end of 45S pre-rRNA has been located on a cloned rDNA fragment from mouse by r-loop mapping and the nuclease S1 protection technique. 45S pre-rRNA could be shown to represent the primary transcript of the ribosomal genes because 5' polyphosphate termini have been detected by an enzymatic assay. The sequence of about 1100 nucleotides surrounding the initiation site for ribosomal RNA transcription has been determined. Features of this region of the ribosomal DNA will be discussed. A comparison of the nucleotide sequence with corresponding areas of ribosomal genes from other eukaryotes does not reveal significant homology in the region of transcription initiation.

MeSH Terms
Animals Base Sequence Cloning, Molecular Coliphages/metabolism DNA Restriction Enzymes DNA, Recombinant/metabolism DNA, Viral/metabolism Endonucleases Mice Nucleic Acid Hybridization Nucleic Acid Precursors/genetics RNA Precursors RNA, Ribosomal/genetics Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic
Chemicals
DNA, Recombinant DNA, Viral Nucleic Acid Precursors RNA Precursors RNA, Ribosomal Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bach R
Grummt I
Allet B
References (21)
21 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.7.1559
Published
1981-04-10
Pages
1559-69
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326781
Subset
IM
Databases
GENBANK
J00625
External Links
PubMed source DOI: 10.1093/nar/9.7.1559 PMC Full Text View journal details
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