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PMID: 6159577 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

DNA-RNA hybrid duplexes containing oligo(dA:rU) sequences are exceptionally unstable and may facilitate termination of transcription.

Nucleic acids research ·Vol. 8 ·No. 10 ·1980-05-24 ·Pages 2295-9

Martin FH, Tinoco I

Abstract

A DNA-RNA hybrid oligonucleotide duplex, dC(pA) 5pG:rC(pU)5pG, which contains a (dA:rU) 5 sequence, is at least 200 times less stable at room temperature than the corresponding duplex containing an (rA:dT) 5 sequence, rC(pA)5pG:dC(pT)5pG. This result provides an explanation for the finding that most primary RNA transcripts terminate in several consecutive rU residues, but not rA residues. It strongly supports the idea that instability of the DNA-RNA hybrid at the growing point of transcription plays a role in termination of transcription.

MeSH Terms

Base Sequence DNA/metabolism Deoxyadenosines Hot Temperature Nucleic Acid Conformation Nucleic Acid Hybridization Oligonucleotides RNA/metabolism Transcription, Genetic Uracil

Chemicals

Deoxyadenosines Oligonucleotides Uracil RNA DNA

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Martin F H

Tinoco I

References (13)

13 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/8.10.2295

Published

1980-05-24

Pages

2295-9

Language

English

Region

England

NLM ID

0411011

PMCID

PMC324078

Subset

Grants

NIGMS NIH HHS · GM 10840 · United States

External Links

PubMed source DOI: 10.1093/nar/8.10.2295 PMC Full Text View journal details

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DNA-RNA hybrid duplexes containing oligo(dA:rU) sequences are exceptionally unstable and may facilitate termination of transcription.

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