Home LiteratureArticle Details
PMID: 6264394 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A T to A base substitution and small deletions in the conalbumin TATA box drastically decrease specific in vitro transcription.

Nucleic acids research ·Vol. 9 ·No. 8 ·1981-04-24 ·Pages 1813-24

Wasylyk B, Chambon P

Abstract

We have previously shown that a T to G transversion at the second T of the conalbumin "TATA" box drastically decreases specific initiation of transcription by RNA polymerase B (1). We now report that a T to A transcription at the same nucleotide also drastically decreases transcription, suggesting that these mutations influence transcription mainly by altering nucleotide recognition rather than helix stability. Small deletions of 2, 3 and 4 nucleotides in the "TATA" box also drastically decrease specific initiation of transcription.

MeSH Terms
Animals Base Composition Base Sequence Conalbumin/genetics DNA Restriction Enzymes DNA, Recombinant/metabolism Egg Proteins/genetics Mutation Plasmids RNA Polymerase II/metabolism Transcription, Genetic
Chemicals
DNA, Recombinant Egg Proteins Conalbumin RNA Polymerase II DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wasylyk B
Chambon P
References (23)
23 references, click to expand
  1. Ubiquitous and gene-specific regulatory 5' sequences in a sea urchin histone DNA clone coding for histone protein variants.
    Nucleic Acids Res. 1980 Mar 11;8(5):957-77 PMID: 7443547
  2. DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3855-9 PMID: 6933441
  3. The effect of bisulfite modification on the template activity of DNA for DNA polymerase I.
    Nucleic Acids Res. 1974 Jul;1(7):889-99 PMID: 10793721
  4. Genetic regulation: the Lac control region.
    Science. 1975 Jan 10;187(4171):27-35 PMID: 1088926
  5. Nucleotide sequence of an RNA polymerase binding site from the DNA of bacteriophage fd.
    Proc Natl Acad Sci U S A. 1975 Feb;72(2):737-41 PMID: 1054851
  6. Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):784-8 PMID: 1093168
  7. Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2170-4 PMID: 209457
  8. pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.
    Nucleic Acids Res. 1978 Aug;5(8):2721-8 PMID: 358147
  9. Contacts between Escherichia coli RNA polymerase and thymines in the lac UV5 promoter.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3233-7 PMID: 386333
  10. Selective and accurate initiation of transcription at the Ad2 major late promotor in a soluble system dependent on purified RNA polymerase II and DNA.
    Cell. 1979 Oct;18(2):469-84 PMID: 498279
  11. Messenger RNA for the Ad2 DNA binding protein: DNA sequences encoding the first leader and heterogenity at the mRNA 5' end.
    Cell. 1979 Oct;18(2):569-80 PMID: 227610
  12. Coding potential and regulatory signals of the polyoma virus genome.
    Nature. 1980 Jan 31;283(5746):445-53 PMID: 6243401
  13. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  14. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  15. Leader sequences of Strongylocentrotus purpuratus histone mRNAs start at a unique heptanucleotide common to all five histone genes.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1265-9 PMID: 6154927
  16. Identification of regulatory sequences in the prelude sequences of an H2A histone gene by the study of specific deletion mutants in vivo.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1432-6 PMID: 6929494
  17. Specific in vitro initiation of transcription on conalbumin and ovalbumin genes and comparison with adenovirus-2 early and late genes.
    Nature. 1980 Jun 5;285(5764):367-73 PMID: 7383157
  18. E. coli RNA polymerase interacts homologously with two different promoters.
    Cell. 1980 Jun;20(2):269-81 PMID: 6248238
  19. Organization and sequence studies of the 17-piece chicken conalbumin gene.
    Nature. 1979 Dec 6;282(5739):567-74 PMID: 551292
  20. Promoter sequences of eukaryotic protein-coding genes.
    Science. 1980 Sep 19;209(4463):1406-14 PMID: 6251548
  21. Accurate transcription initiation on a purified mouse beta-globin DNA fragment in a cell-free system.
    Cell. 1980 Jul;20(3):691-9 PMID: 7418005
  22. Transcriptional fidelity of histone genes injected into Xenopus oocyte nuclei.
    Nature. 1980 Nov 6;288(5786):100-2 PMID: 7432506
  23. Regulation of simian virus 40 early transcription in vitro by a purified tumor antigen.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):5706-10 PMID: 6255460
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.8.1813
Published
1981-04-24
Pages
1813-24
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326805
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/9.8.1813 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2