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PMID: 386333 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Contacts between Escherichia coli RNA polymerase and thymines in the lac UV5 promoter.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 7 ·1979-07-00 ·Pages 3233-7

Simpson RB

Abstract

I have identified those 5 positions of thymines in the lac UV5 promoter that lie close to bound Escherichia coli RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6). Although ultraviolet irradiation of DNA with 5-bromouracil substituted in place of thymine normally cleaves the DNA at the bromouracils, a protein bound to the DNA can perturb these cleavages at those locations at which the protein lies close to the bromine. In the lac promoter most of these contacts lie in three regions. Four contacts lie in the region where transcription initiates; four lie in the "Pribnow box," which is located about 10 base pairs upstream from the initiation site; and three more lie in the "-35 region," located about 35 base pairs upstream from the initiation site. The "Pribnow box" and the "-35 region" are regions whose sequences are partially conserved between promoters and in which most promoter mutations are located; thus, contacts in these two regions probably represent sites of sequence-specific recognition by RNA polymerase.

MeSH Terms
Base Sequence DNA-Directed RNA Polymerases/genetics,metabolism Escherichia coli/genetics Lac Operon Repressor Proteins/metabolism Thymine/metabolism Transcription, Genetic Ultraviolet Rays
Chemicals
Repressor Proteins DNA-Directed RNA Polymerases Thymine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Simpson R B
References (36)
36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.7.3233
Published
1979-07-00
Pages
3233-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383798
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.7.3233 PMC Full Text View journal details
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