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PMID: 377234 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence of the three major early promoters of bacteriophage T7.

Nucleic acids research ·Vol. 6 ·No. 5 ·1979-00-00 ·Pages 1895-907

Siebenlist U

Abstract

I have determined the nucleotide sequences of the three major early promoters of bacteriophage T7 (A1, A2, A3). The sequences confirm the two main homologies found between other known promoters for E. coli RNA polymerase (nucleoside triphosphate:RNA nucleotidyl transferase, E.C. 2. 7. 7. 6). In particular, all three T7 promoters show a very good match with the -35 region homology; the A2 and A3 promoters share a 17 basepair sequence in this region. On the other hand, the match with the Pribnow Box homology is much less pronounced and different for each T7 promoter.

MeSH Terms

Base Sequence Coliphages/metabolism DNA Restriction Enzymes DNA, Viral/metabolism DNA-Directed RNA Polymerases/metabolism Escherichia coli/enzymology Oligodeoxyribonucleotides/analysis Protein Biosynthesis Transcription, Genetic

Chemicals

DNA, Viral Oligodeoxyribonucleotides DNA-Directed RNA Polymerases DNA Restriction Enzymes

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Siebenlist U

References (31)

31 references, click to expand

DNA sequence for a low-level promoter of the lac repressor gene and an 'up' promoter mutation.
Nature. 1978 Aug 24;274(5673):762-5 PMID: 355890
High resolution physical mapping of specific binding sites of Escherichia coli RNA polymerase on the DNA of bacteriophage T7 .
J Mol Biol. 1978 Mar 25;120(1):121-31 PMID: 347089
Use of polylysine for adsorption of nuclei acids and enzymes to electron microscope specimen films.
Proc Natl Acad Sci U S A. 1977 Jun;74(6):2311-5 PMID: 329278
Interaction of RNA polymerase with promoters from bacteriophage fd.
Eur J Biochem. 1977 Mar 15;74(1):107-13 PMID: 300680
Physicochomecial studies on interactions between DNA and RNA polymerase. Isolation and mapping of a T7 DNA fragment containing the early promoters for Escherichia coli RNA polymerase.
Biochemistry. 1976 Dec 28;15(26):5776-83 PMID: 795461
Dual control for transcription of the galactose operon by cyclic AMP and its receptor protein at two interspersed promoters.
Cell. 1977 Nov;12(3):847-54 PMID: 200371
Mapping adenines, guanines, and pyrimidines in RNA.
Nucleic Acids Res. 1977 Aug;4(8):2527-38 PMID: 409999
Nucleotide sequence of the operator-promoter region of the galactose operon of Escherichia coli.
Proc Natl Acad Sci U S A. 1977 Jan;74(1):106-10 PMID: 319453
A new method for sequencing DNA.
Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
Nucleotide sequence of bacteriophage phi X174 DNA.
Nature. 1977 Feb 24;265(5596):687-95 PMID: 870828
DNA base sequence of the po promoter region of phage lamdba.
Nature. 1977 Jan 13;265(5590):117-21 PMID: 834253
Novel properties of a restriction endonuclease isolated from Haemophilus parahaemolyticus.
Proc Natl Acad Sci U S A. 1976 Feb;73(2):293-7 PMID: 1061131
Nucleotide sequence of the rightward operator of phage lambda.
Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 PMID: 1055375
Sequence of a repressor-binding site in the DNA of bacteriophage lamda.
Nature. 1974 Aug 2;250(465):394-7 PMID: 4854243
Transcription of the early region of bacteriophage T7: selective initiation with dinucleotides.
J Mol Biol. 1973 Jun 25;77(2):255-77 PMID: 4587752
Studies of the binding of Escherichia coli RNA polymerase to DNA. I. The role of sigma subunit in site selection.
J Mol Biol. 1972 Sep 28;70(2):157-85 PMID: 4562312
Angular alteration of the DNA helix by E. coli RNA polymerase.
Nat New Biol. 1972 Oct 11;239(93):167-70 PMID: 4573694
Bacteriophage T7 early promoters: nucleotide sequences of two RNA polymerase binding sites.
J Mol Biol. 1975 Dec 15;99(3):419-43 PMID: 765476
Sequence of promoter for coat protein gene of bacteriophage fd.
Nature. 1976 Mar 25;260(5549):297-302 PMID: 1256568
Autoregulation and function of a repressor in bacteriophage lambda.
Science. 1976 Oct 8;194(4261):156-61 PMID: 959843
Interaction of RNA polymerase from Escherichia coli with DNA. Effect of temperature and ionic strength on selection of T7 DNA early promoters.
Eur J Biochem. 1976 Jun 1;65(2):387-93 PMID: 949973
Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter.
Proc Natl Acad Sci U S A. 1975 Mar;72(3):784-8 PMID: 1093168
Genetic regulation: the Lac control region.
Science. 1975 Jan 10;187(4171):27-35 PMID: 1088926
Sequence of the PR promoter of phage lambda.
Nature. 1975 Mar 13;254(5496):118-21 PMID: 1117996
Nucleotide sequence of an RNA polymerase binding site from the DNA of bacteriophage fd.
Proc Natl Acad Sci U S A. 1975 Feb;72(2):737-41 PMID: 1054851
The nucleotide sequence of an RNA polymerase binding site on bacteriophage fd DNA.
Nature. 1975 Feb 6;253(5491):410-4 PMID: 1089210
Recognition sequences of repressor and polymerase in the operators of bacteriophage lambda.
Cell. 1975 Jun;5(2):109-13 PMID: 1095210
Lambda repressor regulates the switch between PR and Prm promoters.
Nature. 1976 Aug 19;262(5570):665-9 PMID: 958438
Lambda repressor turns off transcription of its own gene.
Proc Natl Acad Sci U S A. 1975 Dec;72(12):4785-89 PMID: 1061069
A specific endonuclease from Arthrobacter luteus.
J Mol Biol. 1976 Mar 25;102(1):157-65 PMID: 1271462
The nucleotide sequence preceding an RNA polymerase initiation site on SV40 DNA. Part 2. The sequence of the early strand transcript.
Nucleic Acids Res. 1974 Apr;1(4):595-611 PMID: 10793742

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/6.5.1895

Published

1979-00-00

Pages

1895-907

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327819

Subset

Databases

GENBANK

J02518

External Links

PubMed source DOI: 10.1093/nar/6.5.1895 PMC Full Text View journal details

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Nucleotide sequence of the three major early promoters of bacteriophage T7.

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