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PMID: 6294625 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Multiple Pol I initiation sequences in rDNA spacers of Drosophila melanogaster.

Nucleic acids research ·Vol. 10 ·No. 21 ·1982-11-11 ·Pages 7017-26

Coen ES, Dover GA

Abstract

Sequence analysis of the rDNA nontranscribed spacer (NTS) of D.melanogaster shows that it contains many regions that are perfectly homologous with the Pol I transcription initiation site. Each initiation site homology is contained within a 240 base pair repeated sequence which are organised in a tandem array terminating 140 bases pairs upstream from the transcription initiation site. We discuss the significance of these regions and the maintenance of multiple homologies against the observed high rate of spacer divergence between related Drosophila species. In addition there are two other regions of repetition in the NTS: a 90 base pair periodicity commencing 16 bases downstream from the 3' end of the 28S gene and a number of 340 base pair repeats. A similar complex of different periodicities has been described in Xenopus and may be a general feature of NTS sequences.

MeSH Terms
Animals Base Composition Base Sequence Cloning, Molecular DNA/metabolism DNA Polymerase I/genetics DNA Restriction Enzymes DNA, Ribosomal DNA-Directed DNA Polymerase/genetics Drosophila melanogaster/enzymology,genetics Transcription, Genetic
Chemicals
DNA, Ribosomal DNA DNA Polymerase I DNA-Directed DNA Polymerase DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Coen E S
Dover G A
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27 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.21.7017
Published
1982-11-11
Pages
7017-26
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326982
Subset
IM
Databases
GENBANK
K01526, K01527, K01528
External Links
PubMed source DOI: 10.1093/nar/10.21.7017 PMC Full Text View journal details
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