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PMID: 6179077 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Small nuclear RNAs are encoded in the nontranscribed region of ribosomal spacer DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 10 ·1982-05-00 ·Pages 3106-10

Reichel R, Monstein HJ, Jansen HW, Philipson L, Benecke BJ

Abstract

The structure of in vitro synthesized mouse small nuclear RNA transcribed by RNA polymerase I (snPI RNA) was studied by T1 RNase digestion pattern analysis. The patterns of four different snPI RNA species were different from those of the U1 and U2 RNA species. In addition, the four different snPI RNA species, ranging from 130 to 240 nucleotides in length, yielded almost identical patterns. The snPI RNA molecules hybridized to cloned mouse ribosomal DNA containing the nontranscribed spacer DNA and 45S ribosomal precursor RNA molecules did not compete with this hybridization. Southern blot analysis of fragments from the ribosomal DNA confirmed that snPI RNA species exclusively hybridized to sequences corresponding to the so-called nontranscribed ribosomal spacer region.

MeSH Terms
Animals Genes Genetic Linkage Mice Nucleic Acid Hybridization Oligoribonucleotides/analysis RNA/biosynthesis,genetics RNA Polymerase I/metabolism RNA Polymerase II/genetics RNA, Ribosomal/genetics RNA, Small Nuclear
Chemicals
Oligoribonucleotides RNA, Ribosomal RNA, Small Nuclear RNA RNA Polymerase II RNA Polymerase I
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Reichel R
Monstein H J
Jansen H W
Philipson L
Benecke B J
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.10.3106
Published
1982-05-00
Pages
3106-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346362
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.10.3106 PMC Full Text View journal details
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