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PMID: 2103487 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The beta-tubulin gene family in Zea mays: two differentially expressed beta-tubulin genes.

Plant molecular biology ·Vol. 15 ·No. 6 ·1990-12-00 ·Pages 957-72

Hussey PJ, Haas N, Hunsperger J, Larkin J, Snustad DP, Silflow CD

Abstract

Maize beta-tubulins are encoded by a large multigene family with at least nine members, as determined by Southern blot analysis. Two expressed genes, represented by the beta 1 genomic clone and the beta 2 cDNA clone, were examined in this study. The two genes encode beta-tubulins which show 94% sequence identity at the amino acid level. Maize beta 1 transcript levels were highest in seedling root tip and tissue culture cells, which are both rapidly dividing tissues. No transcripts were detected in non-dividing leaf tissue. In contrast, beta 2 transcripts were present at relatively high levels in tissue culture cells and at lower levels in seedling root tip and leaf tissue. The electrophoretic mobility of the beta 2 polypeptide was examined in relation to the constellation of beta-tubulin polypeptides on two-dimensional gel western blots of a maize pollen total protein extract. No evidence for post-translational modification of the beta-tubulin polypeptides was found in pollen.

MeSH Terms
Base Sequence DNA/genetics Gene Expression Regulation Genes, Plant Microtubules/ultrastructure Molecular Sequence Data Multigene Family Organ Specificity Plant Proteins/biosynthesis,genetics Pollen Seeds Sequence Homology, Nucleic Acid Transcription, Genetic Tubulin/biosynthesis,genetics Zea mays/genetics
Chemicals
Plant Proteins Tubulin DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hussey P J
Department of Genetics and Cell Biology, University of Minnesota, St. Paul.
Haas N
Hunsperger J
Larkin J
Snustad D P
Silflow C D
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00039438
Published
1990-12-00
Pages
957-72
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
X52878, X52879
External Links
PubMed source DOI: 10.1007/BF00039438 View journal details
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