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PMID: 2104804 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of nuclear localizing sequences derived from yeast ribosomal protein L29.

The EMBO journal ·Vol. 9 ·No. 1 ·1990-01-00 ·Pages 91-9

Underwood MR, Fried HM

Abstract

Two particular seven-amino-acid segments from yeast ribosomal protein L29 caused a non-nuclear reporter protein to associate almost exclusively with the yeast nucleus. The two L29-derived nuclear localizing sequences were identical in five of the seven residues, many of which were basic amino acids. Generally, localization of the reporter protein was most impaired by replacement of the basic residues. A particular Arg residue was unique; substitution by any amino acid including Lys diminished nuclear localization of the reporter protein. In L29 the corresponding Arg 25----Lys substitution within the nuclear localizing sequence distal to the N-terminus was without effect, as evidence by normal rates of ribosome assembly and cell growth. However, the analogous Arg 8----Lys substitution within the localizing sequence proximal to the N-terminus led to greatly reduced rates of ribosome assembly and cell growth. Finally, when both localizing sequences contained the Arg----Lys substitution a still greater decrease in ribosome assembly and cell growth was observed. These results were as expected if the two short peptide sequences functioned in nuclear localization and/or assembly of yeast ribosomal protein L29.

MeSH Terms
Amino Acid Sequence Cell Nucleus/metabolism Cloning, Molecular Fluorescent Antibody Technique Molecular Sequence Data Mutation Plasmids RNA Precursors/metabolism RNA, Ribosomal/metabolism Recombinant Fusion Proteins Ribosomal Proteins/genetics,metabolism Ribosomes/metabolism Saccharomyces cerevisiae/analysis,ultrastructure Structure-Activity Relationship beta-Galactosidase/genetics
Chemicals
RNA Precursors RNA, Ribosomal Recombinant Fusion Proteins Ribosomal Proteins ribosomal protein L29 beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Underwood M R
Department of Biochemistry, University of North Carolina, Chapel Hill 27599.
Fried H M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-01-00
Pages
91-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC551634
Subset
IM
Grants
NIGMS NIH HHS · GM37117 · United States
NIGMS NIH HHS · T32-GM07092 · United States
External Links
PubMed source PMC Full Text View journal details
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