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PMID: 21048083 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Rice pollen hybrid incompatibility caused by reciprocal gene loss of duplicated genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 107 ·No. 47 ·2010-11-23 ·Pages 20417-22

Mizuta Y, Harushima Y, Kurata N

Abstract

Genetic incompatibility is a barrier contributing to species isolation and is caused by genetic interactions. We made a whole genome survey of two-way interacting loci acting within the gametophyte or zygote using independence tests of marker segregations in an F(2) population from an intersubspecific cross between O. sativa subspecies indica and japonica. We detected only one reproducible interaction, and identified paralogous hybrid incompatibility genes, DOPPELGANGER1 (DPL1) and DOPPELGANGER2 (DPL2), by positional cloning. Independent disruptions of DPL1 and DPL2 occurred in indica and japonica, respectively. DPLs encode highly conserved, plant-specific small proteins (∼10 kDa) and are highly expressed in mature anther. Pollen carrying two defective DPL alleles became nonfunctional and did not germinate, suggesting an essential role for DPLs in pollen germination. Although rice has many duplicated genes resulting from ancient whole genome duplication, the origin of this gene duplication was in recent small-scale gene duplication, occurring after Oryza-Brachypodium differentiation. Comparative analyses suggested the geographic and phylogenetic distribution of these two defective alleles, showing that loss-of-function mutations of DPL1 genes emerged multiple times in indica and its wild ancestor, O. rufipogon, and that the DPL2 gene defect is specific to japonica cultivars.

MeSH Terms
Base Sequence Chromosome Mapping Cloning, Molecular DNA, Complementary/genetics Gene Components Gene Frequency Genes, Duplicate/genetics Genes, Plant/genetics Genetic Complementation Test Genetic Speciation Genomics/methods Germination/genetics Hybridization, Genetic Immunoblotting In Situ Hybridization Molecular Sequence Data Mutation/genetics Oryza/genetics Pollen/genetics Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, DNA
Chemicals
DNA, Complementary
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mizuta Yoko
Plant Genetics Laboratory, Genetic Strains Research Center, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan.
Harushima Yoshiaki
Kurata Nori
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
DOI
10.1073/pnas.1003124107
Published
2010-11-23
Epub
2010-00-03
Pages
20417-22
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC2996679
Subset
IM
Databases
GENBANK
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External Links
PubMed source DOI: 10.1073/pnas.1003124107 PMC Full Text View journal details
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