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PMID: 2105455 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sequence elements required for activity of a murine major histocompatibility complex class II promoter bind common and cell-type-specific nuclear factors.

Molecular and cellular biology ·Vol. 10 ·No. 2 ·1990-02-00 ·Pages 593-604

Dedrick RL, Jones PP

Abstract

We have examined the sequence elements and corresponding DNA-binding factors required for transient expression of the A alpha d promoter fused to the bacterial chloramphenicol acetyltransferase reporter gene in a variety of cultured cell lines. Deletion analysis demonstrated that only about 110 nucleotides of sequence 5' of the transcription start site are required for constitutive expression in the murine B-lymphoma cell line A20 or for gamma interferon-induced expression in the murine monocytic cell line WEHI-3. Linker-scanner mutation of this region indicated that at least three sequence elements are required for promoter activity. These elements correspond to the conserved sequence elements found in other human and mouse class II genes, the X box, the Y box, and the H box. Analysis of DNA-binding activity showed that the three most predominant factors present in extracts from WEHI-3, A20, or L cells (which do not express the class II genes) are actually a family of factors that bind to a fourth sequence element, overlapping the 3' end of the X-box sequence, that is homologous to the cyclic AMP-responsive enhancer element. A single common factor that binds to the Y box was detected in extracts from all cells tested, as has been seen with the Y-box elements of other class II genes. Another common factor was found that binds to the more conserved 5' region of the X-box element, although A20 extracts contained a second, distinct binding activity for this region. A common binding factor for the H-box element was detected in extracts from WEHI-3 and L cells. However, this activity was absent in A20 cell extracts. Instead, two different H-box-binding activities were detected, suggesting that different components are involved in class II gene expression in B cells and macrophages. Finally, gamma interferon treatment did not significantly alter the DNA-binding activity in WEHI-3 cells for any of the sequence elements shown to be required for induced chloramphenicol acetyltransferase expression.

MeSH Terms
Animals B-Lymphocytes/immunology Base Sequence Cell Line Chloramphenicol O-Acetyltransferase/genetics Chromosome Deletion DNA-Binding Proteins/metabolism Gene Expression Regulation Genes, MHC Class II/drug effects Interferon-gamma/pharmacology Lymphoma Mice Molecular Sequence Data Mutation Nuclear Proteins/metabolism Plasmids Promoter Regions, Genetic Recombinant Proteins Transfection
Chemicals
DNA-Binding Proteins Nuclear Proteins Recombinant Proteins Interferon-gamma Chloramphenicol O-Acetyltransferase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dedrick R L
Department of Biological Sciences, Stanford University, California 94305.
Jones P P
References (59)
59 references, click to expand
  1. Increased expression of Ia antigens on resting B cells: an additional role for B-cell growth factor.
    Proc Natl Acad Sci U S A. 1984 Oct;81(19):6149-53 PMID: 6435125
  2. Multiple regulatory regions on the 5' side of the mouse E alpha gene.
    Proc Natl Acad Sci U S A. 1988 May;85(9):3075-9 PMID: 3129725
  3. Opposing effects of glucocorticoids on interferon-gamma-induced murine macrophage Fc receptor and Ia antigen expression.
    J Immunol. 1985 Apr;134(4):2462-9 PMID: 3919097
  4. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  5. Coordinate induction of Ia alpha, beta, and Ii mRNA in a macrophage cell line.
    J Immunol. 1985 Jul;135(1):632-6 PMID: 3923113
  6. Complete nucleotide sequence of a functional HLA-DP beta gene and the region between the DP beta 1 and DP alpha 1 genes: comparison of the 5' ends of HLA class II genes.
    Nucleic Acids Res. 1985 Mar 11;13(5):1607-21 PMID: 2987832
  7. Gene organization of DC and DX subregions of the human major histocompatibility complex.
    Proc Natl Acad Sci U S A. 1985 May;82(10):3410-4 PMID: 3858830
  8. Antagonistic effect of interferon-beta on the interferon-gamma-induced expression of Ia antigen in murine macrophages.
    J Immunol. 1985 Sep;135(3):1857-63 PMID: 3926890
  9. Reactivation by a trans-acting factor of human major histocompatibility complex Ia gene expression in interspecies hybrids between an Ia-negative human B-cell variant and an Ia-positive mouse B-cell lymphoma.
    Proc Natl Acad Sci U S A. 1985 Aug;82(15):5145-9 PMID: 3875096
  10. T-lymphocyte recognition of antigen in association with gene products of the major histocompatibility complex.
    Annu Rev Immunol. 1985;3:237-61 PMID: 2415139
  11. Effect of serotonin on murine macrophages: suppression of Ia expression by serotonin and its reversal by 5-HT2 serotonergic receptor antagonists.
    J Immunol. 1986 Jul 1;137(1):276-82 PMID: 2423604
  12. Regulation of human interleukin-2 gene: functional DNA sequences in the 5' flanking region for the gene expression in activated T lymphocytes.
    Cell. 1986 Aug 1;46(3):401-5 PMID: 3015413
  13. Multiple nuclear factors interact with the immunoglobulin enhancer sequences.
    Cell. 1986 Aug 29;46(5):705-16 PMID: 3091258
  14. Effects of interferon-gamma and tumor necrosis factor-alpha on the expression of an Ia antigen on a murine macrophage cell line.
    J Immunol. 1986 Nov 1;137(9):2853-6 PMID: 3093585
  15. Regulation of a transfected human class II major histocompatibility complex gene in human fibroblasts.
    Proc Natl Acad Sci U S A. 1986 Dec;83(23):9139-43 PMID: 3097644
  16. Firefly luciferase gene: structure and expression in mammalian cells.
    Mol Cell Biol. 1987 Feb;7(2):725-37 PMID: 3821727
  17. Compartmentalization of MHC class II gene expression in transgenic mice.
    Cell. 1988 May 6;53(3):357-70 PMID: 3259162
  18. Identification of a nuclear factor that binds to a conserved sequence of the I-A beta gene.
    J Immunol. 1988 Jun 1;140(11):3995-4002 PMID: 3131425
  19. Congenital immunodeficiency with a regulatory defect in MHC class II gene expression lacks a specific HLA-DR promoter binding protein, RF-X.
    Cell. 1988 Jun 17;53(6):897-906 PMID: 3133120
  20. An enhancer factor defect in a mutant Burkitt lymphoma cell line.
    J Exp Med. 1988 Jun 1;167(6):1781-90 PMID: 3133441
  21. Transcriptional activation of HLA-DR alpha by interferon gamma requires a trans-acting protein.
    Proc Natl Acad Sci U S A. 1988 Jul;85(13):4672-6 PMID: 3133656
  22. Characterization of the cDNA encoding a protein binding to the major histocompatibility complex class II Y box.
    Proc Natl Acad Sci U S A. 1988 Oct;85(19):7322-6 PMID: 3174636
  23. Distinct cloned class II MHC DNA binding proteins recognize the X box transcription element.
    Science. 1988 Oct 7;242(4875):69-71 PMID: 3140376
  24. B-cell control region at the 5' end of a major histocompatibility complex class II gene: sequences and factors.
    Mol Cell Biol. 1988 Oct;8(10):3975-87 PMID: 3141781
  25. B-cell-specific and interferon-gamma-inducible regulation of the HLA-DR alpha gene.
    Proc Natl Acad Sci U S A. 1988 Nov;85(22):8598-602 PMID: 3141929
  26. Detailed delineation of an interferon-gamma-responsive element important in human HLA-DRA gene expression in a glioblastoma multiform line.
    Proc Natl Acad Sci U S A. 1988 Nov;85(22):8618-22 PMID: 2847168
  27. Evidence for a trans-acting factor that regulates the transcription of class II major histocompatibility complex genes: genetic and functional analysis.
    Proc Natl Acad Sci U S A. 1988 Dec;85(23):8830-4 PMID: 3143110
  28. Differences in DNA sequence specificity among MHC class II X box binding proteins.
    J Immunol. 1989 Feb 1;142(3):1005-14 PMID: 2492326
  29. Anatomy of a new B-cell-specific enhancer.
    Mol Cell Biol. 1989 Jan;9(1):303-11 PMID: 2467189
  30. New B-lymphocyte-specific enhancer-binding protein.
    Mol Cell Biol. 1989 Jan;9(1):312-20 PMID: 2564635
  31. Class II box consensus sequences in the HLA-DR alpha gene: transcriptional function and interaction with nuclear proteins.
    Mol Cell Biol. 1989 Jan;9(1):50-6 PMID: 2538724
  32. Influence of the major histocompatibility complex on positive thymic selection of V beta 17a+ T cells.
    Science. 1989 Apr 14;244(4901):214-7 PMID: 2784868
  33. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
  34. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  35. NF-kappa B binds within a region required for B-cell-specific expression of major histocompatibility complex class II gene E alpha d.
    Mol Cell Biol. 1989 Feb;9(2):844-6 PMID: 2496303
  36. Transcriptional control signals of a eukaryotic protein-coding gene.
    Science. 1982 Jul 23;217(4557):316-24 PMID: 6283634
  37. Prostaglandins modulate macrophage Ia expression.
    Nature. 1982 Sep 9;299(5879):163-5 PMID: 6287286
  38. Corticosteroids inhibit murine macrophage Ia expression and interleukin 1 production.
    J Immunol. 1982 Nov;129(5):1803-5 PMID: 6811653
  39. Regulation of murine macrophage Ia antigen expression by an immune interferon-like lymphokine: inhibitory effect of endotoxin.
    J Immunol. 1982 Dec;129(6):2402-6 PMID: 6815267
  40. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
    Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
  41. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  42. The murine E alpha immune response gene.
    Cell. 1983 Mar;32(3):745-54 PMID: 6403249
  43. B cell activation. III. B cell plasma membrane depolarization and hyper-Ia antigen expression induced by receptor immunoglobulin cross-linking are coupled.
    J Exp Med. 1983 Nov 1;158(5):1589-99 PMID: 6415207
  44. Role of aberrant HLA-DR expression and antigen presentation in induction of endocrine autoimmunity.
    Lancet. 1983 Nov 12;2(8359):1115-9 PMID: 6138647
  45. Characterization of enhancer elements in the long terminal repeat of Moloney murine sarcoma virus.
    J Virol. 1984 Jan;49(1):183-9 PMID: 6690710
  46. The class II molecules of the human and murine major histocompatibility complex.
    Cell. 1984 Jan;36(1):1-13 PMID: 6198089
  47. Structure of the 5' ends of immunoglobulin genes: a novel conserved sequence.
    Proc Natl Acad Sci U S A. 1984 May;81(9):2650-4 PMID: 6425835
  48. High level transient expression of a chloramphenicol acetyl transferase gene by DEAE-dextran mediated DNA transfection coupled with a dimethyl sulfoxide or glycerol shock treatment.
    Nucleic Acids Res. 1984 Jul 25;12(14):5707-17 PMID: 6589587
  49. HLA class II induction in human islet cells by interferon-gamma plus tumour necrosis factor or lymphotoxin.
    Nature. 1987 Mar 19-25;326(6110):304-6 PMID: 3102976
  50. T cell tolerance by clonal elimination in the thymus.
    Cell. 1987 Apr 24;49(2):273-80 PMID: 3494522
  51. Hyperinducibility of Ia antigen on astrocytes correlates with strain-specific susceptibility to experimental autoimmune encephalomyelitis.
    Proc Natl Acad Sci U S A. 1987 Jun;84(12):4219-23 PMID: 3495802
  52. Class II genes of the human major histocompatibility complex. The DO beta gene is a divergent member of the class II beta gene family.
    J Biol Chem. 1987 Jun 25;262(18):8759-66 PMID: 3036827
  53. Distinct mechanisms regulate MHC class II gene expression in B cells and macrophages.
    J Immunol. 1987 Aug 1;139(3):942-8 PMID: 3110290
  54. Sequence-specific interactions of nuclear factors with conserved sequences of human class II major histocompatibility complex genes.
    Proc Natl Acad Sci U S A. 1987 Jul;84(14):4939-43 PMID: 3037529
  55. A multiplicity of CCAAT box-binding proteins.
    Cell. 1987 Sep 11;50(6):863-72 PMID: 3476205
  56. Conserved major histocompatibility complex class II boxes--X and Y--are transcriptional control elements and specifically bind nuclear proteins.
    Proc Natl Acad Sci U S A. 1987 Sep;84(17):6249-53 PMID: 3114745
  57. Transgenic mice selectively lacking MHC class II (I-E) antigen expression on B cells: an in vivo approach to investigate Ia gene function.
    Cell. 1987 Oct 23;51(2):175-87 PMID: 3499228
  58. Norepinephrine inhibits gamma-interferon-induced major histocompatibility class II (Ia) antigen expression on cultured astrocytes via beta-2-adrenergic signal transduction mechanisms.
    Proc Natl Acad Sci U S A. 1988 Feb;85(4):1292-6 PMID: 2829222
  59. Immunoglobulin heavy-chain enhancer requires one or more tissue-specific factors.
    Science. 1985 Jan 18;227(4684):266-70 PMID: 3917575
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.2.593-604.1990
Published
1990-02-00
Pages
593-604
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360848
Subset
IM
Grants
NIAID NIH HHS · AI 07290 · United States
NIAID NIH HHS · AI 19512 · United States
NIAID NIH HHS · AI 19964 · United States
External Links
PubMed source DOI: 10.1128/mcb.10.2.593-604.1990 PMC Full Text View journal details
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