Home LiteratureArticle Details
PMID: 2106068 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Serum stimulation of the c-fos enhancer induces reversible changes in c-fos chromatin structure.

Molecular and cellular biology ·Vol. 10 ·No. 3 ·1990-03-00 ·Pages 1126-33

Feng JL, Villeponteau B

Abstract

Transcription of the proto-oncogene c-fos is known to be activated by growth factors in serum and subsequently repressed by the Fos protein. We show that generalized DNase I sensitivity of c-fos chromatin correlates closely with enhancer activity during induction, repression, and superinduction of the c-fos gene. Within 90 s of serum stimulation, proximal DNA sequences on both sides of the enhancer exhibit increased DNase I sensitivity. Within 5 min, elevated DNase I sensitivity spreads to chromatin at the distal 3' end of the c-fos gene. These results suggest that an open state of chromatin is propagated in both directions from the enhancer. The induced alterations in chromatin structure precede the increased transcriptional activity of the c-fos gene, suggesting that these changes in chromatin structure potentiate transcription.

MeSH Terms
Cell Division Chromatin/ultrastructure Culture Media Deoxyribonuclease I/pharmacology Enhancer Elements, Genetic Gene Expression Regulation HeLa Cells Humans In Vitro Techniques Proto-Oncogene Mas Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-fos Restriction Mapping Time Factors Transcription, Genetic
Chemicals
Chromatin Culture Media MAS1 protein, human Proto-Oncogene Mas Proto-Oncogene Proteins Proto-Oncogene Proteins c-fos Deoxyribonuclease I
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Feng J L
Department of Biological Chemistry, University of Michigan, Ann Arbor 48109-2007.
Villeponteau B
References (32)
32 references, click to expand
  1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  2. Nuclease hypersensitive sites in chromatin.
    Annu Rev Biochem. 1988;57:159-97 PMID: 3052270
  3. Steroid receptors: elements for modulation of eukaryotic transcription.
    Annu Rev Biochem. 1976;45:721-46 PMID: 183602
  4. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 PMID: 73185
  5. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  6. Hb switching in chickens.
    Cell. 1980 Apr;19(4):973-80 PMID: 6155217
  7. Commitment of chick oviduct tubular gland cells to produce ovalbumin mRNA during hormonal withdrawal and restimulation.
    J Cell Biol. 1980 Oct;87(1):142-51 PMID: 7419588
  8. Isolation and characterization of the complete chicken beta-globin gene region: frequent deletion of the adult beta-globin genes in lambda.
    Nucleic Acids Res. 1981 Aug 11;9(15):3731-46 PMID: 6269092
  9. Definition of 5' and 3' structural boundaries of the chromatin domain containing the ovalbumin multigene family.
    J Biol Chem. 1982 Feb 10;257(3):1501-7 PMID: 6276388
  10. Chromatin structure of the chicken beta-globin gene region. Sensitivity to DNase I, micrococcal nuclease, and DNase II.
    J Biol Chem. 1982 Jul 10;257(13):7730-6 PMID: 6282852
  11. FBJ murine osteosarcoma virus: identification and molecular cloning of biologically active proviral DNA.
    J Virol. 1982 Nov;44(2):674-82 PMID: 6292525
  12. Simultaneous analysis of conformation and transcription of A and B groups of vitellogenin genes in male and female Xenopus during primary and secondary activation by estrogen.
    Nucleic Acids Res. 1983 Feb 25;11(4):1151-66 PMID: 6687495
  13. Nuclease sensitivity and DNA methylation in estrogen regulation of Xenopus laevis vitellogenin gene expression.
    J Biol Chem. 1983 Jul 25;258(14):8908-14 PMID: 6306003
  14. Molecular biology. Enhancers, chromosome position effects, and transgenic mice.
    Nature. 1983 Nov 24-30;306(5941):313-4 PMID: 6646213
  15. Expression of a microinjected immunoglobulin gene in the spleen of transgenic mice.
    Nature. 1983 Nov 24-30;306(5941):332-6 PMID: 6417544
  16. DNase I sensitive domain of the gene coding for the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase.
    Biochemistry. 1984 May 8;23(10):2309-14 PMID: 6329271
  17. Reversible and persistent changes in chromatin structure accompany activation of a glucocorticoid-dependent enhancer element.
    Cell. 1984 Aug;38(1):29-38 PMID: 6088072
  18. Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene.
    Nature. 1984 Oct 4-10;311(5985):433-8 PMID: 6090941
  19. Torsional stress promotes the DNAase I sensitivity of active genes.
    Cell. 1984 Dec;39(3 Pt 2):469-78 PMID: 6096006
  20. Induction of c-fos gene and protein by growth factors precedes activation of c-myc.
    Nature. 1984 Dec 20-1985 Jan 2;312(5996):716-20 PMID: 6334806
  21. Assembly and propagation of repressed and depressed chromosomal states.
    Cell. 1985 Oct;42(3):705-11 PMID: 2996773
  22. Transient accumulation of c-fos RNA following serum stimulation requires a conserved 5' element and c-fos 3' sequences.
    Cell. 1985 Oct;42(3):889-902 PMID: 2414012
  23. Identification of a transcriptional enhancer element upstream from the proto-oncogene fos.
    Science. 1985 Dec 6;230(4730):1174-7 PMID: 3865371
  24. The human argininosuccinate synthetase locus and citrullinemia.
    Adv Hum Genet. 1986;15:161-96, 291-2 PMID: 3513483
  25. The use of psoralen-modified DNA to probe the mechanism of enhancer action.
    Cell. 1986 May 23;45(4):567-74 PMID: 3011273
  26. The DNase I sensitive domain of the chicken lysozyme gene spans 24 kb.
    Nucleic Acids Res. 1986 Aug 11;14(15):6085-99 PMID: 3748804
  27. Effect of protein synthesis inhibitors on growth factor activation of c-fos, c-myc, and actin gene transcription.
    Mol Cell Biol. 1986 Apr;6(4):1050-7 PMID: 2431274
  28. Chromatin structural transitions and the phenomenon of vitellogenin gene memory in chickens.
    Mol Cell Biol. 1986 Jun;6(6):1886-93 PMID: 3785183
  29. Gamma rays and bleomycin nick DNA and reverse the DNase I sensitivity of beta-globin gene chromatin in vivo.
    Mol Cell Biol. 1987 May;7(5):1917-24 PMID: 2439900
  30. Transcriptional autoregulation of the proto-oncogene fos.
    Nature. 1988 Jul 28;334(6180):314-9 PMID: 2839774
  31. Requirement for fos gene expression in the transcriptional activation of collagenase by other oncogenes and phorbol esters.
    Cell. 1988 Jul 29;54(3):325-34 PMID: 2840203
  32. Chromosomal subunits in active genes have an altered conformation.
    Science. 1976 Sep 3;193(4256):848-56 PMID: 948749
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.3.1126-1133.1990
Published
1990-03-00
Pages
1126-33
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360979
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.10.3.1126-1133.1990 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2