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PMID: 2107074 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Beta-lactam antibiotic biosynthetic genes have been conserved in clusters in prokaryotes and eukaryotes.

The EMBO journal ·Vol. 9 ·No. 3 ·1990-03-00 ·Pages 741-7

Smith DJ, Burnham MK, Bull JH, Hodgson JE, Ward JM, Browne P, Brown J, Barton B, Earl AJ, Turner G

Abstract

A cosmid clone containing closely linked beta-lactam antibiotic biosynthetic genes was isolated from a gene library of Flavobacterium sp. SC 12,154. The location within the cluster of the DNA thought to contain the gene for delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase (ACVS), the first step in the beta-lactam antibiotic biosynthetic pathway, was identified by a novel method. This DNA facilitated the isolation, by cross-hybridization, of the corresponding DNA from Streptomyces clavuligerus ATCC 27064, Penicillium chrysogenum Oli13 and Aspergillus nidulans R153. Evidence was obtained which confirmed that the cross-hybridizing sequences contained the ACVS gene. In each case the ACVS gene was found to be closely linked to other beta-lactam biosynthetic genes and constituted part of a gene cluster.

MeSH Terms
Anti-Bacterial Agents/biosynthesis Aspergillus nidulans/genetics Cloning, Molecular Cosmids DNA, Bacterial/genetics,isolation & purification DNA, Fungal/genetics,isolation & purification Flavobacterium/genetics Gene Library Genes, Bacterial Genes, Fungal Multigene Family Nucleic Acid Hybridization Penicillium/genetics Penicillium chrysogenum/genetics Peptide Synthases/genetics Restriction Mapping Sequence Homology, Nucleic Acid Species Specificity Streptomyces/genetics beta-Lactams
Chemicals
Anti-Bacterial Agents DNA, Bacterial DNA, Fungal beta-Lactams Peptide Synthases alpha-aminoadipyl-cysteinyl-valine synthetase
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Smith D J
Department of Microbiology, University of Bristol, UK.
Burnham M K
Bull J H
Hodgson J E
Ward J M
Browne P
Brown J
Barton B
Earl A J
Turner G
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-03-00
Pages
741-7
Language
English
Region
England
NLM ID
8208664
PMCID
PMC551730
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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