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PMID: 2108134 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Alkaline induction of a novel gene locus, alx, in Escherichia coli.

Journal of bacteriology ·Vol. 172 ·No. 4 ·1990-04-00 ·Pages 2184-6

Bingham RJ, Hall KS, Slonczewski JL

Abstract

A novel pH-regulated locus inducible over 100-fold in alkaline media was identified in Escherichia coli through screening of 93,000 Mu dI1734 (lacZ Kmr) operon fusions at pH 6.5 and pH 8.5. Four lacZ fusions that showed expression only at the higher pH were mapped at 67.5 min by P1 transduction crosses. The locus was designated alx.

MeSH Terms
Crosses, Genetic Escherichia coli/genetics Gene Expression Regulation, Bacterial Genes, Bacterial Hydrogen-Ion Concentration Kinetics Operon Recombinant Fusion Proteins/biosynthesis Transduction, Genetic beta-Galactosidase/biosynthesis,genetics
Chemicals
Recombinant Fusion Proteins beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bingham R J
Department of Biology, Kenyon College, Gambier, Ohio 43022.
Hall K S
Slonczewski J L
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20 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.4.2184-2186.1990
Published
1990-04-00
Pages
2184-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208722
Subset
IM
Grants
NIGMS NIH HHS · GM38585 · United States
External Links
PubMed source DOI: 10.1128/jb.172.4.2184-2186.1990 PMC Full Text View journal details
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