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PMID: 2111442 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Constitutive and interleukin-1 (IL-1)-inducible factors interact with the IL-1-responsive element in the IL-6 gene.

Molecular and cellular biology ·Vol. 10 ·No. 6 ·1990-06-00 ·Pages 2757-64

Isshiki H, Akira S, Tanabe O, Nakajima T, Shimamoto T, Hirano T, Kishimoto T

Abstract

The interleukin-6 (IL-6) promoter is rapidly and transiently activated with other cytokines, including IL-1, tumor necrosis factor, and platelet-derived growth factor, as well as phorbol esters and agents that increase intracellular cyclic AMP. In this study, we have investigated cis-acting regulatory elements and trans-acting factors responsible for IL-1-induced IL-6 gene expression. Studies on the 5' deletion mutants of the human IL-6 gene suggested that the IL-1-responsive element was mapped within the IL-6 promoter region (-180 to -123) which was homologous to the c-fos serum-responsive enhancer element. Gel retardation assay identified two types of nuclear factors that bound to this region, one constitutive and the other inducible. These two factors recognized a 14-base-pair (bp) palindromic sequence, ACATTGCACAATCT. Furthermore, three copies of this 14-bp palindrome conferred IL-1 responsiveness to the basal enhancerless IL-6 promoter, indicating that a 14-bp-dyad symmetry sequence was an IL-1-responsive element in the IL-6 gene.

MeSH Terms
Animals Base Sequence Cell Line Cell Nucleus/metabolism Enhancer Elements, Genetic Gene Expression/drug effects Glioma Humans Interleukin-1/pharmacology Interleukin-6/genetics L Cells/immunology Methylation Mice Molecular Sequence Data Promoter Regions, Genetic/drug effects Protein-Tyrosine Kinases/genetics Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-fos RNA, Messenger/drug effects,genetics Sequence Homology, Nucleic Acid Transcription, Genetic/drug effects Transfection
Chemicals
Interleukin-1 Interleukin-6 Proto-Oncogene Proteins Proto-Oncogene Proteins c-fos RNA, Messenger Protein-Tyrosine Kinases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Isshiki H
Division of Cellular Immunology, Osaka University, Osaka, Japan.
Akira S
Tanabe O
Nakajima T
Shimamoto T
Hirano T
Kishimoto T
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.6.2757-2764.1990
Published
1990-06-00
Pages
2757-64
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360636
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.10.6.2757-2764.1990 PMC Full Text View journal details
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