Home LiteratureArticle Details
PMID: 2116418 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The yeast RNA1 gene product necessary for RNA processing is located in the cytosol and apparently excluded from the nucleus.

The Journal of cell biology ·Vol. 111 ·No. 2 ·1990-08-00 ·Pages 309-21

Hopper AK, Traglia HM, Dunst RW

Abstract

The yeast RNA1 gene is required for RNA processing and nuclear transport of RNA. The rna1-1 mutation of this locus causes defects in pre-tRNA splicing, processing of the primary pre-rRNA transcript, production of mRNA and export of RNA from the nucleus to the cytosol. To understand how this gene product can pleiotropically affect these processes, we sought to determine the intracellular location of the RNA1 protein. As determined by indirect immunofluorescence localization and organelle fractionation, the RNA1 antigen is found exclusively or primarily in the cytoplasm. Only a tiny fraction of the endogenous protein could be localized to and functional in the nucleus. Furthermore, the RNA1 antigen does not localize differently under stress conditions. These findings suggest that the RNA1 protein is not directly involved in RNA processing but may modify nuclear proteins or otherwise transmit a signal from the cytosol to the nucleus or play a role in maintaining the integrity of the nucleus.

MeSH Terms
Cell Nucleus/ultrastructure Cytosol/ultrastructure Fluorescent Antibody Technique Fungal Proteins/analysis,genetics GTPase-Activating Proteins Genes, Fungal RNA Precursors/genetics RNA Processing, Post-Transcriptional RNA, Fungal/genetics RNA, Messenger/genetics Recombinant Fusion Proteins/analysis Restriction Mapping Saccharomyces cerevisiae/genetics,ultrastructure Saccharomyces cerevisiae Proteins beta-Galactosidase/analysis,genetics
Chemicals
Fungal Proteins GTPase-Activating Proteins RNA Precursors RNA, Fungal RNA, Messenger RNA1 protein, S cerevisiae Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hopper A K
Department of Biological Chemistry, M. S. Hershey Medical Center, Pennsylvania State University, Hershey 17033.
Traglia H M
Dunst R W
References (47)
47 references, click to expand
  1. Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae.
    Genes Dev. 1989 Aug;3(8):1206-16 PMID: 2676722
  2. Synthesis of ribosomes in Saccharomyces cerevisiae.
    Microbiol Rev. 1989 Jun;53(2):256-71 PMID: 2666845
  3. Yeast mutation thought to arrest mRNA transport markedly increases the length of the 3' poly(A) on polyadenylated RNA.
    J Mol Biol. 1989 Aug 20;208(4):697-700 PMID: 2478712
  4. Cytoplasmic anchoring proteins and the control of nuclear localization.
    Cell. 1989 Dec 22;59(6):949-51 PMID: 2688899
  5. Split tRNA genes and their products: a paradigm for the study of cell function and evolution.
    Yeast. 1989 Nov-Dec;5(6):405-27 PMID: 2694676
  6. Nuclear pre-mRNA splicing in yeast.
    Yeast. 1989 Nov-Dec;5(6):439-57 PMID: 2694677
  7. Structural and functional analyses of Saccharomyces cerevisiae wild-type and mutant RNA1 genes.
    Mol Cell Biol. 1989 Jul;9(7):2989-99 PMID: 2674676
  8. PRP4 (RNA4) from Saccharomyces cerevisiae: its gene product is associated with the U4/U6 small nuclear ribonucleoprotein particle.
    Mol Cell Biol. 1989 Sep;9(9):3698-709 PMID: 2528686
  9. PRP4: a protein of the yeast U4/U6 small nuclear ribonucleoprotein particle.
    Mol Cell Biol. 1989 Sep;9(9):3710-9 PMID: 2528687
  10. Macromolecule synthesis in temperature-sensitive mutants of yeast.
    J Bacteriol. 1967 May;93(5):1662-70 PMID: 5337848
  11. Temperature-sensitive yeast mutant defective in ribonucleic acid production.
    J Bacteriol. 1969 Sep;99(3):807-14 PMID: 5370278
  12. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  13. Macromolecule synthesis and breakdown in relation to sporulation and meiosis in yeast.
    J Bacteriol. 1974 Aug;119(2):619-28 PMID: 4604714
  14. The role of cytoplasmic membranes in controlling the transport of nuclear messenger RNA and initiation of protein synthesis.
    Proc Natl Acad Sci U S A. 1974 Jul;71(7):2658-62 PMID: 4605329
  15. Immunoaffinity chromatography of proteins.
    Methods Enzymol. 1974;34:723-31 PMID: 4141422
  16. A yeast mutant which accumulates precursor tRNAs.
    Cell. 1978 Jun;14(2):211-9 PMID: 352536
  17. Transcription and processing of intervening sequences in yeast tRNA genes.
    Cell. 1978 Jun;14(2):221-36 PMID: 352537
  18. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  19. Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments.
    Gene. 1979 Dec;8(1):17-24 PMID: 395030
  20. Processing of intervening sequences: a new yeast mutant which fails to excise intervening sequences from precursor tRNAs.
    Cell. 1980 Mar;19(3):741-51 PMID: 7363329
  21. The effect of temperature-sensitive RNA mutants on the transcription products from cloned ribosomal protein genes of yeast.
    Cell. 1981 Jun;24(3):679-86 PMID: 7018694
  22. The genes for fifteen ribosomal proteins of Saccharomyces cerevisiae.
    J Biol Chem. 1981 Oct 10;256(19):10176-83 PMID: 6268628
  23. The organization and transcription of the galactose gene cluster of Saccharomyces.
    J Mol Biol. 1981 Oct 25;152(2):285-315 PMID: 6276569
  24. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  25. A suppressor of temperature-sensitive rna mutations that affect mRNA metabolism in Saccharomyces cerevisiae.
    Mol Cell Biol. 1982 May;2(5):571-77 PMID: 7050675
  26. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
  27. Open reading frame expression vectors: a general method for antigen production in Escherichia coli using protein fusions to beta-galactosidase.
    Proc Natl Acad Sci U S A. 1983 Jul;80(14):4432-6 PMID: 6308625
  28. Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces.
    J Cell Biol. 1984 Mar;98(3):922-33 PMID: 6365930
  29. Targeting of E. coli beta-galactosidase to the nucleus in yeast.
    Cell. 1984 Apr;36(4):1057-65 PMID: 6323016
  30. Intracellular targeting and import of an F1-ATPase beta-subunit-beta-galactosidase hybrid protein into yeast mitochondria.
    Proc Natl Acad Sci U S A. 1984 Jul;81(13):3983-7 PMID: 6330727
  31. Amino terminus of the yeast GAL4 gene product is sufficient for nuclear localization.
    Proc Natl Acad Sci U S A. 1984 Oct;81(19):5951-5 PMID: 6091123
  32. Characterization of an essential Saccharomyces cerevisiae gene related to RNA processing: cloning of RNA1 and generation of a new allele with a novel phenotype.
    Mol Cell Biol. 1985 May;5(5):907-15 PMID: 3889591
  33. Saccharomyces cerevisiae tRNA ligase. Purification of the protein and isolation of the structural gene.
    J Biol Chem. 1986 Feb 25;261(6):2978-86 PMID: 3512545
  34. Splicing of messenger RNA precursors.
    Annu Rev Biochem. 1986;55:1119-50 PMID: 2943217
  35. The heat-shock response.
    Annu Rev Biochem. 1986;55:1151-91 PMID: 2427013
  36. Identification and nuclear localization of yeast pre-messenger RNA processing components: RNA2 and RNA3 proteins.
    J Cell Biol. 1986 Dec;103(6 Pt 1):2103-12 PMID: 3536958
  37. Protein import into the cell nucleus.
    Annu Rev Cell Biol. 1986;2:367-90 PMID: 3548772
  38. Cloning and characterization of LOS1, a Saccharomyces cerevisiae gene that affects tRNA splicing.
    Mol Cell Biol. 1987 Mar;7(3):1208-16 PMID: 3031485
  39. Transfer RNA modification.
    Annu Rev Biochem. 1987;56:263-87 PMID: 3304135
  40. A trans-acting suppressor restores splicing of a yeast intron with a branch point mutation.
    Genes Dev. 1987 Jul;1(5):445-55 PMID: 2890553
  41. Identification of a yeast snRNP protein and detection of snRNP-snRNP interactions.
    Cell. 1987 Dec 24;51(6):1019-26 PMID: 2961458
  42. Splicing of yeast nuclear pre-mRNA in vitro requires a functional 40S spliceosome and several extrinsic factors.
    Genes Dev. 1987 Mar;1(1):7-18 PMID: 3322937
  43. Amino acid sequences that determine the nuclear localization of yeast histone 2B.
    Mol Cell Biol. 1987 Nov;7(11):4048-57 PMID: 3123916
  44. Regulated overproduction of the GAL4 gene product greatly increases expression from galactose-inducible promoters on multi-copy expression vectors in yeast.
    Gene. 1987;61(2):123-33 PMID: 2832257
  45. Activation of DNA-binding activity in an apparently cytoplasmic precursor of the NF-kappa B transcription factor.
    Cell. 1988 Apr 22;53(2):211-7 PMID: 3129195
  46. RNA11 protein is associated with the yeast spliceosome and is localized in the periphery of the cell nucleus.
    Mol Cell Biol. 1988 Jun;8(6):2379-93 PMID: 3043176
  47. N2,N2-dimethylguanosine-specific tRNA methyltransferase contains both nuclear and mitochondrial targeting signals in Saccharomyces cerevisiae.
    J Cell Biol. 1989 Oct;109(4 Pt 1):1411-9 PMID: 2677019
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.111.2.309
Published
1990-08-00
Pages
309-21
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2116204
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.111.2.309 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2