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PMID: 2120198 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

DNA sequence of the purC gene encoding 5'-phosphoribosyl-5-aminoimidazole-4-N-succinocarboxamide synthetase and organization of the dapA-purC region of Escherichia coli K-12.

Journal of bacteriology ·Vol. 172 ·No. 10 ·1990-10-00 ·Pages 6035-41

Tiedeman AA, DeMarini DJ, Parker J, Smith JM

Abstract

5'-Phosphoribosyl-5-aminoimidazole-4-N-succinocarboxamide synthetase (EC 6.3.2.6), encoded by the purC gene of Escherichia coli K-12, catalyzes the synthesis of 5'-phosphoribosyl-5-aminoimidazole-4-N-succinocarboxamide from 5'-phosphoribosyl-5-aminoimidazole-4-carboxylic acid. The mature protein, as deduced from the purC structural gene sequence, contains 237 amino acids and has a calculated Mr of 26,998. The control region of the purC gene was identified by primer extension mapping of the 5' end of the purC mRNA. The purC control region contains a binding site for and is regulated by the purine repressor, the product of the purR gene. An unusual feature of the 5' untranslated region of the purC mRNA is the presence of a repetitive extragenic palindrome sequence normally found in intercistronic or 3' untranslated regions. The DNA sequence was extended 1.281 kilobases upstream of the purC structural gene and overlapped with the previously determined dapA sequence. Termination of transcription from the dapA-purC intercistronic region may occur within the -35 region of the purC control region. The purC gene has been positioned on the E. coli restriction map and is transcribed in a counterclockwise direction.

MeSH Terms
Amino Acid Sequence Bacillus subtilis/enzymology,genetics Base Sequence DNA, Bacterial/genetics Escherichia coli/enzymology,genetics Genes, Bacterial Introns Molecular Sequence Data Oligonucleotide Probes Peptide Synthases/genetics Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
DNA, Bacterial Oligonucleotide Probes Peptide Synthases phosphoribosylaminoimidazole-succinocarboxamide synthetase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tiedeman A A
Seattle Biomedical Research Institute, Washington 98109.
DeMarini D J
Parker J
Smith J M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.10.6035-6041.1990
Published
1990-10-00
Pages
6035-41
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC526926
Subset
IM
Grants
NIAID NIH HHS · AI20068 · United States
NIGMS NIH HHS · GM25855 · United States
NCRR NIH HHS · RR-01685 · United States
Databases
GENBANK
M33928
External Links
PubMed source DOI: 10.1128/jb.172.10.6035-6041.1990 PMC Full Text View journal details
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