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PMID: 2982787 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli.

Journal of bacteriology ·Vol. 161 ·No. 3 ·1985-03-00 ·Pages 1219-21

Winans SC, Elledge SJ, Krueger JH, Walker GC

Abstract

A mutation of a cloned gene that has been made by introducing a transposon or some other selectable genetic determinant can be crossed into the gene's original replicon by linearizing the cloned DNA and transforming a recB recC sbcB mutant. A number of applications of this method are described with genes of either chromosomal or plasmid origin.

MeSH Terms
Chromosome Deletion Cloning, Molecular DNA Transposable Elements DNA, Recombinant Escherichia coli/genetics Genetic Engineering Rec A Recombinases/genetics Recombination, Genetic Transformation, Genetic
Chemicals
DNA Transposable Elements DNA, Recombinant Rec A Recombinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Winans S C
Elledge S J
Krueger J H
Walker G C
References (21)
21 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.161.3.1219-1221.1985
Published
1985-03-00
Pages
1219-21
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215030
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.161.3.1219-1221.1985 PMC Full Text View journal details
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