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PMID: 3881396 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Identification of pKM101-encoded loci specifying potentially lethal gene products.

Journal of bacteriology ·Vol. 161 ·No. 1 ·1985-01-00 ·Pages 417-24

Winans SC, Walker GC

Abstract

Two pKM101-encoded loci (designated kilA and kilB) have been identified which elaborate products that are potentially lethal to the bacterial cell. The lethal effects of each of these products is inhibited by two other plasmid-encoded loci, designated korA and korB (for kil override). Both korA and korB are required to control the lethality of either kil gene. In the presence of korA and korB both kil genes have other phenotypes: kilB is necessary for conjugal transfer, whereas kilA is responsible for the small-colony morphology on defined media that is characteristic of pKM101-containing strains (the Slo phenotype).

MeSH Terms
Conjugation, Genetic DNA, Bacterial/genetics DNA, Recombinant Genes, Bacterial Genes, Lethal Klebsiella pneumoniae/genetics Mutation Phenotype Plasmids Temperature Transformation, Bacterial
Chemicals
DNA, Bacterial DNA, Recombinant
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Winans S C
Walker G C
References (25)
25 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.161.1.417-424.1985
Published
1985-01-00
Pages
417-24
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214887
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.161.1.417-424.1985 PMC Full Text View journal details
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