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PMID: 6270504 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Restriction endonuclease cleavage map of pKM101: relationship to parental plasmid R46.

Molecular & general genetics : MGG ·Vol. 182 ·No. 2 ·1981-00-00 ·Pages 268-72

Langer PJ, Walker GC

Abstract

A detailed restriction endonuclease cleavage map of the plasmid pKM101 has been constructed. pKM101 plasmids containing individual Tn5 insertions were used to facilitate the ordering of restriction fragments generated by enzymes cleaving pKM101 at multiple sites. By restriction enzyme analysis, pKM101 (35.4 kilobases) appears to have arisen from its clinically-isolated parent by deletion of a single DNA region which codes for three of the four drug resistances carried by R46.

MeSH Terms
Base Composition Chromosome Deletion Chromosome Mapping DNA Restriction Enzymes/metabolism DNA, Bacterial/genetics Drug Resistance, Microbial Escherichia coli/genetics Phenotype Plasmids
Chemicals
DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Langer P J
Walker G C
References (20)
20 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00269669
Published
1981-00-00
Pages
268-72
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NCI NIH HHS · CA21615 · United States
NIGMS NIH HHS · GM07287 · United States
External Links
PubMed source DOI: 10.1007/BF00269669
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