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PMID: 377021 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mutagenesis and repair deficiencies of Escherichia coli umuC mutants are suppressed by the plasmid pKM101.

Molecular & general genetics : MGG ·Vol. 172 ·No. 1 ·1979-04-17 ·Pages 17-24

Walker GC, Dobson PP

Abstract

The presence of the drug resistance plasmid pKM101 restored the ability of Escherichia coli umuC mutant strains to be mutated by methyl methanesulfonate. Inducible (Weigle) reactivation of ultraviolet-irradiated bacteriophage lambda was not observed in uvrA6 umuC mutant strains lacking pKM101 but was observed if the plasmid was present in the strains. In a uvrA+ umuC36 strain pKM101 increased the efficiency of the Weigle reactivation process. Plasmid-mediated UV-resistance and plasmid-mediated phage reactivation were observed in umuC(pKM101) strains both in uvrA+ and uvrA6 backgrounds. No restoration of methyl methanesulfonate mutability by pKM101 was observed in umuC36 recA56 strains. pKM101 mutants unable to enhance mutagenesis in umuC+ backgrounds also had no effect on methyl methanesulfonate mutagenesis in umuC mutant strains. Neither a umuC mutation nor the presence of pKM101 affected the UV induction of protein X, the recA protein. Hypotheses relating the mode of action of pKM101 to the process of mutagenesis and inducible phage reactivation are discussed.

MeSH Terms
DNA Repair/radiation effects Escherichia coli/genetics,radiation effects Methyl Methanesulfonate/pharmacology Mutagens Mutation/radiation effects Phenotype R Factors Ultraviolet Rays
Chemicals
Mutagens Methyl Methanesulfonate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Walker G C
Dobson P P
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35 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00276210
Published
1979-04-17
Pages
17-24
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00276210
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