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PMID: 2123556 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence and promoter analysis of SPO13, a meiosis-specific gene of Saccharomyces cerevisiae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 23 ·1990-12-00 ·Pages 9406-10

Buckingham LE, Wang HT, Elder RT, McCarroll RM, Slater MR, Esposito RE

Abstract

The SPO13 gene, required for meiosis I segregation in Saccharomyces cerevisiae, produces two developmentally regulated transcripts (1.0 and 1.4 kilobases) that differ in length at their 5' ends. The shorter transcript is sufficient to complement the spo13-1 mutation and contains a major open reading frame encoding a highly basic protein of 33.4 kilodaltons. A fragment upstream (-170 to -8) of the open reading frame confers meiosis-specific transcription on a spo13-HIS3 fusion. Deletions at the 5' end of spo13-lacZ fusions define a region between -140 and -80 that is essential for meiosis-specific expression. This region acts in an orientation-independent manner and is responsive to the MAT-RME regulatory cascade. It contains a 10-base-pair sequence, TAGCCGCCGA, found in a number of meiosis-specific genes, that appears to be required for SPO13 expression. This sequence is identical to URS1, a ubiquitous mitotic repressor element.

Related Genes
HOP1 MER1 RED1 SPO11 SPO13 SPO16
MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Fungal/genetics Genes, Fungal Meiosis Molecular Sequence Data Mutagenesis, Site-Directed Plasmids Promoter Regions, Genetic Saccharomyces cerevisiae/genetics,growth & development,physiology Sequence Homology, Nucleic Acid Spores, Fungal/physiology beta-Galactosidase/genetics,metabolism
Chemicals
DNA, Fungal beta-Galactosidase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Buckingham L E
Department of Molecular Genetics and Cell Biology, University of Chicago, IL 60637.
Wang H T
Elder R T
McCarroll R M
Slater M R
Esposito R E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.23.9406
Published
1990-12-00
Pages
9406-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC55174
Subset
IM
Grants
NCI NIH HHS · CA09273 · United States
NIGMS NIH HHS · F32 GM11798 · United States
NIGMS NIH HHS · GM07183-10 · United States
Databases
GENBANK
M38357
External Links
PubMed source DOI: 10.1073/pnas.87.23.9406 PMC Full Text View journal details
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