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PMID: 21258325 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Transcriptome-wide sequencing reveals numerous APOBEC1 mRNA-editing targets in transcript 3' UTRs.

Nature structural & molecular biology ·Vol. 18 ·No. 2 ·2011-02-00 ·Pages 230-6

Rosenberg BR, Hamilton CE, Mwangi MM, Dewell S, Papavasiliou FN

Abstract

Apolipoprotein B-editing enzyme, catalytic polypeptide-1 (APOBEC1) is a cytidine deaminase initially identified by its activity in converting a specific cytidine (C) to uridine (U) in apolipoprotein B (apoB) mRNA transcripts in the small intestine. Editing results in the translation of a truncated apoB isoform with distinct functions in lipid transport. To address the possibility that APOBEC1 edits additional mRNAs, we developed a transcriptome-wide comparative RNA sequencing (RNA-Seq) screen. We identified and validated 32 previously undescribed mRNA targets of APOBEC1 editing, all of which are located in AU-rich segments of transcript 3' untranslated regions (3' UTRs). Further analysis established several characteristic sequence features of editing targets, which were predictive for the identification of additional APOBEC1 substrates. The transcriptomics approach to RNA editing presented here dramatically expands the list of APOBEC1 mRNA editing targets and reveals a novel cellular mechanism for the modification of transcript 3' UTRs.

MeSH Terms
3' Untranslated Regions APOBEC-1 Deaminase Animals Base Sequence Cytidine Deaminase/genetics Gene Expression Profiling Mice Mice, Inbred C57BL RNA Editing RNA, Messenger/genetics
Chemicals
3' Untranslated Regions RNA, Messenger APOBEC-1 Deaminase Apobec1 protein, mouse Cytidine Deaminase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Rosenberg Brad R
Laboratory of Lymphocyte Biology, The Rockefeller University, New York, New York, USA.
Hamilton Claire E
Mwangi Michael M
Dewell Scott
Papavasiliou F Nina
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Article Info
Journal
Nature structural & molecular biology
Abbr.
Nat Struct Mol Biol
ISSN
1545-9985
DOI
10.1038/nsmb.1975
Published
2011-02-00
Epub
2011-00-23
Pages
230-6
Language
English
Region
United States
NLM ID
101186374
PMCID
PMC3075553
Subset
IM
Grants
NCI NIH HHS · R01 CA098495-06A2 · United States
NIGMS NIH HHS · GM07739 · United States
NCRR NIH HHS · UL1RR024143 · United States
NCRR NIH HHS · UL1 RR024143 · United States
NCI NIH HHS · R01 CA098495 · United States
NIGMS NIH HHS · T32 GM007739 · United States
Corrections
ErratumIn
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External Links
PubMed source DOI: 10.1038/nsmb.1975 PMC Full Text View journal details
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