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PMID: 212606 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Heteroduplex analysis of the sequence relations between the RNAs of mink cell focus-inducing and murine leukemia viruses.

Journal of virology ·Vol. 28 ·No. 1 ·1978-10-00 ·Pages 352-60

Chien YH, Verma IM, Shih TY, Scolnick EM, Davidson N

Abstract

The sequence relationships betwen AKR ecotropic virus and an AKR-derived "mink cell focus-inducing" (MCF) isolate (AKR MCF 247), between Moloney murine leukemia virus (M-MLV) and an M-MLV MCF isolate (M-MLV83), and between AKR and M-MLV were studied by electron microscopic heteroduplex analysis. The MCF-specific sequences were found to map from 1.95 kilobases (kb) to 2.75 kb (+/- 0.15 kb) from the 3' end of the RNAs for both MCF isolates. The major sequence nonhomology regions between AKR and M-MLV lie between 0.9 and 3.5 kb from the 3' end. However, the AKR and M-MLV sequences immediately adjacent to the 1.95- and 2.75-kb junctions with MCF-specific sequences are relatively similar in AKR and M-MLV. Our results suggest that the env gene of MLVs maps from 1 kb to 3 kb from the 3' end of the genomic RNA and that the carboxyl end of the glycoprotein of each MCF strain is similar (or identical) to that of its ecotropic parent.

MeSH Terms
AKR murine leukemia virus/analysis,growth & development Animals Base Sequence Cell Line Leukemia Virus, Murine/analysis Mink Moloney murine leukemia virus/analysis,growth & development Nucleic Acid Conformation Nucleic Acid Hybridization RNA, Viral/analysis Recombination, Genetic
Chemicals
RNA, Viral
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Chien Y H
Verma I M
Shih T Y
Scolnick E M
Davidson N
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23 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.28.1.352-360.1978
Published
1978-10-00
Pages
352-60
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC354274
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.28.1.352-360.1978 PMC Full Text View journal details
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