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PMID: 56459 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Purification and serological characterization of the major envelope glycoprotein from AKR murine leukemia virus and its reactivity with autogenous immune sera from mice.

Journal of virology ·Vol. 17 ·No. 3 ·1976-03-00 ·Pages 727-36

Ihle JN, Denny TP, Bolognesi DP

Abstract

The major envelope glycoprotein (gp71) from AKR murine leukemia virus (MuLV) was purified and its serological reactivity with heterologous and autogenous immune mouse sera was examined. Homologous and interspecies competition radioimmunoassays using antisera to Rauscher-MulV gp69/71 or Friend-MuLV gp71 or antisera to feline leukemia virus to precipitate 125I-labeled gp71 from various MuLV showed that distinct differences exist between Rauscher- or Friend-MuLV and AKR-MuLV glycoproteins. Characteristically the AKR-MuLV gp71, in contrast to FLV or RLV gp71, does not compete fully in homologous or interspecies radioimmunoassays with iodinated Friend of Rauscher glycoproteins. Purified 125I-labeled AKR-MuLV gp71, in contrast to the Rauscher- or Friend-MuLV glycoproteins, reacts with normal (autogenous immune) mouse sera in direct radioimmune precipitation assays. Competition experiments further demonstrate that this is a predominant immunological reactivity of normal mouse sera which had previously been detected by radioimmune precipitation assay against intact virions.

MeSH Terms
AKR murine leukemia virus/analysis,immunology Animals Cross Reactions Epitopes Glycoproteins/immunology,isolation & purification Immune Sera Leukemia Virus, Feline/immunology Leukemia Virus, Murine/analysis Mice/immunology Molecular Weight Rauscher Virus/immunology Viral Proteins/immunology,isolation & purification
Chemicals
Epitopes Glycoproteins Immune Sera Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ihle J N
Denny T P
Bolognesi D P
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24 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.17.3.727-736.1976
Published
1976-03-00
Pages
727-36
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515471
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.17.3.727-736.1976 PMC Full Text View journal details
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