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PMID: 4373739 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Expression of murine leukemia virus structural antigens on the surface of chemically induced murine sarcomas.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 12 ·1974-12-00 ·Pages 5037-41

Grant JP, Bigner DD, Fischinger PJ, Bolognesi DP

Abstract

Cultured cells of different chemically-induced C57BL/6N murine sarcomas produced variable amounts of infectious murine leukemia virus (MuLV) and contained proportional amounts of MuLV structural components as determined by radioimmunoassay. Monospecific antisera directed against the major MuLV glycoprotein (gp71), the major internal antigen (p30), and the ribonucleoprotein (p10) were capable of mediating tumor cell lysis in the presence of complement, suggesting that these viral structural components were localized at least in part to the cell surface. Membrane immunofluorescence studies with MuLV p30 antiserum confirmed surface localization. Addition of MuLV p30 polypeptide to normal cells and tumor cells enhanced the cytotoxicity of MuLV p30 antiserum. Studies are presented which suggest that the presence of MuLV structural components on cell surfaces can be independent of virus production and cellular transformation.

MeSH Terms
Animals Antibodies, Viral Antigens, Viral/analysis Carcinogens Cell Line Cell Membrane/immunology Complement System Proteins Cytotoxicity Tests, Immunologic Fibroblasts Leukemia Virus, Murine/immunology Methylcholanthrene Mice Peptides/immunology Radioimmunoassay Sarcoma, Experimental/chemically induced,immunology,microbiology
Chemicals
Antibodies, Viral Antigens, Viral Carcinogens Peptides Methylcholanthrene Complement System Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Grant J P
Bigner D D
Fischinger P J
Bolognesi D P
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.12.5037
Published
1974-12-00
Pages
5037-41
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC434035
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.12.5037 PMC Full Text View journal details
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