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PMID: 2129286 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The puff-specific Drosophila protein Bj6, encoded by the gene no-on transient A, shows homology to RNA-binding proteins.

Chromosoma ·Vol. 100 ·No. 1 ·1990-12-00 ·Pages 37-47

von Besser H, Schnabel P, Wieland C, Fritz E, Stanewsky R, Saumweber H

Abstract

The DNA coding for the puff-specific protein Bj6 has been isolated by expression cloning. The gene is localized in 14C1,2 on the X chromosome and is expressed ubiquitously during embryonic development with prominent expression during the first 12 h of embryogenesis. cDNA and genomic clones have been sequenced and show a single open-reading frame of 2.1 kb length, coding for a Mr = 77,000 basic protein. In the aminoterminal half of the protein we detect stretches of repeated amino acids, centrally a region with homology to RNA-binding proteins containing the RNP 1 and RNP 2 consensus motif of RNA binding proteins, and the carboxyterminal part is rich in charged amino acids. The Bj6 protein is a product of the gene no-on transient A, a gene required for normal vision and courtship behaviour in Drosophila.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Carrier Proteins/genetics Cloning, Molecular Consensus Sequence Drosophila/embryology,genetics Drosophila Proteins Molecular Sequence Data Nuclear Proteins/genetics,metabolism Nucleic Acid Conformation Nucleotide Mapping Open Reading Frames RNA-Binding Proteins Repetitive Sequences, Nucleic Acid Restriction Mapping Sequence Homology, Nucleic Acid X Chromosome
Chemicals
Carrier Proteins Drosophila Proteins NONA protein, Drosophila Nuclear Proteins RNA-Binding Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
von Besser H
Institut für Entwicklungsphysiologie Universität zu Köln, Federal Republic of Germany.
Schnabel P
Wieland C
Fritz E
Stanewsky R
Saumweber H
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00337601
Published
1990-12-00
Pages
37-47
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
Databases
GENBANK
X55902
External Links
PubMed source DOI: 10.1007/BF00337601 View journal details
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