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PMID: 21339329 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

The yeast Cbk1 kinase regulates mRNA localization via the mRNA-binding protein Ssd1.

The Journal of cell biology ·Vol. 192 ·No. 4 ·2011-02-21 ·Pages 583-98

Kurischko C, Kim HK, Kuravi VK, Pratzka J, Luca FC

Abstract

The mRNA-binding protein Ssd1 is a substrate for the Saccharomyces cerevisiae LATS/NDR orthologue Cbk1, which controls polarized growth, cell separation, and cell integrity. We discovered that most Ssd1 localizes diffusely within the cytoplasm, but some transiently accumulates at sites of polarized growth. Cbk1 inhibition and cellular stress cause Ssd1 to redistribute to mRNA processing bodies (P-bodies) and stress granules, which are known to repress translation. Ssd1 recruitment to P-bodies is independent of mRNA binding and is promoted by the removal of Cbk1 phosphorylation sites. SSD1 deletion severely impairs the asymmetric localization of the Ssd1-associated mRNA, SRL1. Expression of phosphomimetic Ssd1 promotes polarized localization of SRL1 mRNA, whereas phosphorylation-deficient Ssd1 causes constitutive localization of SRL1 mRNA to P-bodies and causes cellular lysis. These data support the model that Cbk1-mediated phosphorylation of Ssd1 promotes the cortical localization of Ssd1-mRNA complexes, whereas Cbk1 inhibition, cellular stress, and Ssd1 dephosphorylation promote Ssd1-mRNA interactions with P-bodies and stress granules, leading to translational repression.

MeSH Terms
Binding Sites Cell Enlargement Cell Polarity Cytoplasm/metabolism Gene Deletion Intracellular Signaling Peptides and Proteins/genetics,metabolism,physiology Phosphorylation Protein Serine-Threonine Kinases/genetics,metabolism,physiology RNA, Messenger/analysis,metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism,physiology
Chemicals
Intracellular Signaling Peptides and Proteins RNA, Messenger Saccharomyces cerevisiae Proteins Ssd1 protein, S cerevisiae CBK1 protein, S cerevisiae Protein Serine-Threonine Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kurischko Cornelia
Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Kim Hong Kyung
Kuravi Venkata K
Pratzka Juliane
Luca Francis C
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
1540-8140
DOI
10.1083/jcb.201011061
Published
2011-02-21
Pages
583-98
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC3044126
Subset
IM
Grants
NIGMS NIH HHS · R01 GM060575 · United States
NIGMS NIH HHS · R01-GM-060575 · United States
External Links
PubMed source DOI: 10.1083/jcb.201011061 PMC Full Text View journal details
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