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PMID: 21368824 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Copy number variation and selection during reprogramming to pluripotency.

Nature ·Vol. 471 ·No. 7336 ·2011-03-03 ·Pages 58-62

Hussein SM, Batada NN, Vuoristo S, Ching RW, Autio R, Närvä E, Ng S, Sourour M, Hämäläinen R, Olsson C, Lundin K, Mikkola M, Trokovic R, Peitz M, Brüstle O, Bazett-Jones DP, Alitalo K, Lahesmaa R, Nagy A, Otonkoski T

Abstract

The mechanisms underlying the low efficiency of reprogramming somatic cells into induced pluripotent stem (iPS) cells are poorly understood. There is a clear need to study whether the reprogramming process itself compromises genomic integrity and, through this, the efficiency of iPS cell establishment. Using a high-resolution single nucleotide polymorphism array, we compared copy number variations (CNVs) of different passages of human iPS cells with their fibroblast cell origins and with human embryonic stem (ES) cells. Here we show that significantly more CNVs are present in early-passage human iPS cells than intermediate passage human iPS cells, fibroblasts or human ES cells. Most CNVs are formed de novo and generate genetic mosaicism in early-passage human iPS cells. Most of these novel CNVs rendered the affected cells at a selective disadvantage. Remarkably, expansion of human iPS cells in culture selects rapidly against mutated cells, driving the lines towards a genetic state resembling human ES cells.

MeSH Terms
Cell Line Cellular Reprogramming/genetics Chromosome Fragile Sites/genetics DNA Copy Number Variations/genetics Embryonic Stem Cells/cytology,metabolism Fibroblasts/cytology,metabolism Haplotypes/genetics Humans In Situ Hybridization, Fluorescence Induced Pluripotent Stem Cells/cytology,metabolism,pathology Mosaicism Mutagenesis/genetics Oligonucleotide Array Sequence Analysis Polymorphism, Single Nucleotide/genetics Selection, Genetic/genetics
Authors & Affiliations
20 authors, click to expand affiliations / ORCID
Hussein Samer M
Samuel Lunenfeld Research Institute, Toronto, Ontario M5T 3H7, Canada.
Batada Nizar N
Vuoristo Sanna
Ching Reagan W
Autio Reija
Närvä Elisa
Ng Siemon
Sourour Michel
Hämäläinen Riikka
Olsson Cia
Lundin Karolina
Mikkola Milla
Trokovic Ras
Peitz Michael
Brüstle Oliver
Bazett-Jones David P
Alitalo Kari
Lahesmaa Riitta
Nagy Andras
Otonkoski Timo
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Article Info
Journal
Nature
Abbr.
Nature
ISSN
1476-4687
Published
2011-03-03
Pages
58-62
Language
English
Region
England
NLM ID
0410462
Subset
IM
Databases
GEO
Corrections
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