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PMID: 2138058 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cell-free protein sorting to the regulated and constitutive secretory pathways.

Cell ·Vol. 60 ·No. 5 ·1990-03-09 ·Pages 837-47

Tooze SA, Huttner WB

Abstract

To elucidate the mechanism of secretory granule formation, we here identify the first intermediate in this process, the immature secretory granule, in the neuroendocrine cell line PC12 and demonstrate the packaging of a regulated secretory protein, secretogranin II, to immature secretory granules in a cell-free system. The formation of immature secretory granules was as fast (t1/2 approximately 5 min) as that of constitutive secretory vesicles identified by the presence of a rapidly secreted heparan sulfate proteoglycan. Using the cell-free system, the formation of post-Golgi secretory vesicles was found to be dependent upon ATP. Two distinct populations of vesicles were formed: immature secretory granules containing secretogranin II and constitutive secretory vesicles containing the heparan sulfate proteoglycan. These results show that in a cell-free system, a constitutive and a regulated secretory protein are sorted upon exit from the trans-Golgi network.

MeSH Terms
Adenosine Triphosphate/metabolism Adrenal Gland Neoplasms Animals Cell Line Cell-Free System Chondroitin Sulfate Proteoglycans/genetics Cytoplasmic Granules/metabolism,ultrastructure Glycosaminoglycans/genetics Golgi Apparatus/metabolism,ultrastructure Heparan Sulfate Proteoglycans Heparitin Sulfate/genetics Kinetics Microscopy, Electron Pheochromocytoma Protein Processing, Post-Translational Proteoglycans/genetics
Chemicals
Chondroitin Sulfate Proteoglycans Glycosaminoglycans Heparan Sulfate Proteoglycans Proteoglycans Adenosine Triphosphate Heparitin Sulfate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tooze S A
European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
Huttner W B
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/0092-8674(90)90097-x
Published
1990-03-09
Pages
837-47
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC7125605
Subset
IM
External Links
PubMed source DOI: 10.1016/0092-8674(90)90097-x PMC Full Text View journal details
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