Home LiteratureArticle Details
PMID: 3243273 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Release of putative exocytic transport vesicles from perforated MDCK cells.

The EMBO journal ·Vol. 7 ·No. 13 ·1988-12-20 ·Pages 4075-85

Bennett MK, Wandinger-Ness A, Simons K

Abstract

Mechanically perforated MDCK cells were used to study membrane transport between the trans-Golgi network and the apical and basolateral plasma membrane domains in vitro. Three membrane transport markers--an apical protein (fowl plague virus haemagglutinin), a basolateral protein (vesicular stomatitis virus G protein), and a lipid marker destined for both domains (C6-NBD-sphingomyelin)--were each accumulated in the trans-Golgi by a 20 degrees C block of transport and their behaviour monitored following cell perforation and incubation at 37 degrees C. In the presence of ATP and in the absence of calcium ions a considerable fraction of the transport markers were released from the perforated cells in sealed membrane vesicles. Control experiments showed that the vesicles were not generated by non-specific vesiculation of the Golgi complex or the plasma membrane. The vesicles had well defined sedimentation properties and the orientation expected of transport vesicles derived from the trans-Golgi network.

MeSH Terms
Animals Biological Transport, Active Biomarkers Cell Line Cell Membrane/metabolism Centrifugation, Density Gradient Exocytosis Golgi Apparatus/metabolism Intracellular Membranes/metabolism
Chemicals
Biomarkers
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bennett M K
European Molecular Biology Laboratory, Heidelberg, FRG.
Wandinger-Ness A
Simons K
References (55)
55 references, click to expand
  1. Immunocytochemical localization of galactosyltransferase in HeLa cells: codistribution with thiamine pyrophosphatase in trans-Golgi cisternae.
    J Cell Biol. 1982 Apr;93(1):223-9 PMID: 6121819
  2. A rapid method of total lipid extraction and purification.
    Can J Biochem Physiol. 1959 Aug;37(8):911-7 PMID: 13671378
  3. Pathway of vesicular stomatitis virus entry leading to infection.
    J Mol Biol. 1982 Apr 15;156(3):609-31 PMID: 6288961
  4. Distribution of clathrin and spiny-coated vesicles on membranes within mature Golgi apparatus elements of mouse liver.
    Eur J Cell Biol. 1982 Aug;28(1):130-8 PMID: 6127212
  5. The cleavage site of the hemagglutinin of fowl plague virus.
    Virology. 1982 Oct 15;122(1):186-90 PMID: 7135832
  6. Sphingolipid metabolism in cultured fibroblasts: microscopic and biochemical studies employing a fluorescent ceramide analogue.
    Proc Natl Acad Sci U S A. 1983 May;80(9):2608-12 PMID: 6573674
  7. Influenza virus hemagglutinin expression is polarized in cells infected with recombinant SV40 viruses carrying cloned hemagglutinin DNA.
    Cell. 1983 Jun;33(2):435-43 PMID: 6305510
  8. Reduced temperature prevents transfer of a membrane glycoprotein to the cell surface but does not prevent terminal glycosylation.
    Cell. 1983 Aug;34(1):233-43 PMID: 6883510
  9. Viral glycoproteins destined for apical or basolateral plasma membrane domains traverse the same Golgi apparatus during their intracellular transport in doubly infected Madin-Darby canine kidney cells.
    J Cell Biol. 1984 Apr;98(4):1304-19 PMID: 6325468
  10. Viruses budding from either the apical or the basolateral plasma membrane domain of MDCK cells have unique phospholipid compositions.
    EMBO J. 1982;1(7):847-52 PMID: 6329709
  11. Transcytosis of the G protein of vesicular stomatitis virus after implantation into the apical plasma membrane of Madin-Darby canine kidney cells. I. Involvement of endosomes and lysosomes.
    J Cell Biol. 1984 Sep;99(3):796-82 PMID: 6088557
  12. Reconstitution of the transport of protein between successive compartments of the Golgi measured by the coupled incorporation of N-acetylglucosamine.
    Cell. 1984 Dec;39(2 Pt 1):405-16 PMID: 6498939
  13. Sorting of an apical plasma membrane glycoprotein occurs before it reaches the cell surface in cultured epithelial cells.
    J Cell Biol. 1984 Dec;99(6):2131-9 PMID: 6501415
  14. Sequential intermediates in the pathway of intercompartmental transport in a cell-free system.
    Cell. 1984 Dec;39(3 Pt 2):525-36 PMID: 6096009
  15. Expression of preprosomatostatin in heterologous cells: biosynthesis, posttranslational processing, and secretion of mature somatostatin.
    Cell. 1984 Dec;39(3 Pt 2):547-55 PMID: 6150766
  16. Intracellular processing of the Newcastle disease virus fusion glycoprotein.
    J Virol. 1985 Mar;53(3):851-7 PMID: 3838349
  17. Lines of BPV-transformed murine cells that constitutively express influenza virus hemagglutinin.
    EMBO J. 1985 Jan;4(1):91-103 PMID: 2990897
  18. Intracellular sorting and basolateral appearance of the G protein of vesicular stomatitis virus in Madin-Darby canine kidney cells.
    J Cell Biol. 1985 Aug;101(2):470-6 PMID: 2991300
  19. Exit of newly synthesized membrane proteins from the trans cisterna of the Golgi complex to the plasma membrane.
    J Cell Biol. 1985 Sep;101(3):949-64 PMID: 2863275
  20. Demonstration of an extensive trans-tubular network continuous with the Golgi apparatus stack that may function in glycosylation.
    Cell. 1985 Nov;43(1):287-95 PMID: 3000603
  21. Reconstitution of an endocytic fusion event in a cell-free system.
    Cell. 1985 Dec;43(3 Pt 2):643-52 PMID: 4075403
  22. A novel prefusion complex formed during protein transport between Golgi cisternae in a cell-free system.
    J Biol Chem. 1986 Feb 15;261(5):2202-7 PMID: 3003101
  23. Microinjected antibodies against the cytoplasmic domain of vesicular stomatitis virus glycoprotein block its transport to the cell surface.
    EMBO J. 1986 May;5(5):931-41 PMID: 3013626
  24. The trans Golgi network: sorting at the exit site of the Golgi complex.
    Science. 1986 Oct 24;234(4775):438-43 PMID: 2945253
  25. ATP-coupled transport of vesicular stomatitis virus G protein between the endoplasmic reticulum and the Golgi.
    J Biol Chem. 1986 Nov 5;261(31):14681-9 PMID: 3021750
  26. ATP-coupled transport of vesicular stomatitis virus G protein. Functional boundaries of secretory compartments.
    J Biol Chem. 1986 Nov 5;261(31):14690-6 PMID: 3021751
  27. A cell-free assay for the insertion of a viral glycoprotein into the plasma membrane.
    J Cell Biol. 1986 Nov;103(5):1829-35 PMID: 3782285
  28. A vesicular intermediate in the transport of hepatoma secretory proteins from the rough endoplasmic reticulum to the Golgi complex.
    J Cell Biol. 1987 Feb;104(2):221-30 PMID: 3027103
  29. Reconstitution of vesicle fusions occurring in endocytosis with a cell-free system.
    EMBO J. 1986 Dec 1;5(12):3091-101 PMID: 3028771
  30. Reconstitution of transport of vesicular stomatitis virus G protein from the endoplasmic reticulum to the Golgi complex using a cell-free system.
    J Cell Biol. 1987 Mar;104(3):749-60 PMID: 3029144
  31. Fusion between endocytic vesicles in a cell-free system.
    Proc Natl Acad Sci U S A. 1987 Mar;84(5):1137-41 PMID: 3469657
  32. Proteolytic maturation of insulin is a post-Golgi event which occurs in acidifying clathrin-coated secretory vesicles.
    Cell. 1987 Jun 19;49(6):865-8 PMID: 3555846
  33. Semi-intact cells permeable to macromolecules: use in reconstitution of protein transport from the endoplasmic reticulum to the Golgi complex.
    Cell. 1987 Aug 14;50(4):523-34 PMID: 3038335
  34. An antibody specific for an endoproteolytic cleavage site provides evidence that pro-opiomelanocortin is packaged into secretory granules in AtT20 cells before its cleavage.
    J Cell Biol. 1987 Jul;105(1):155-62 PMID: 3038924
  35. Purification and characterization of constitutive secretory vesicles from yeast.
    J Cell Biol. 1987 Jul;105(1):163-74 PMID: 3301865
  36. Transport of the vesicular stomatitis glycoprotein to trans Golgi membranes in a cell-free system.
    J Biol Chem. 1987 Sep 15;262(26):12502-10 PMID: 3040752
  37. Perforated MDCK cells support intracellular transport.
    EMBO J. 1987 Aug;6(8):2241-7 PMID: 3665874
  38. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples.
    Anal Biochem. 1978 Jun 15;87(1):206-10 PMID: 98070
  39. Membrane assembly: synthesis and intracellular processing of the vesicular stomatitis viral glycoprotein.
    J Supramol Struct. 1977;7(3-4):353-70 PMID: 211348
  40. Transmembrane biogenesis of the vesicular stomatitis virus glycoprotein.
    J Cell Biol. 1979 Feb;80(2):416-26 PMID: 222771
  41. Demonstration of protein asymmetries in the plasma membrane of cultured renal (MDCK) epithelial cells by lactoperoxidase-mediated iodination.
    FEBS Lett. 1979 Sep 15;105(2):201-4 PMID: 488350
  42. Polarized distribution of viral envelope proteins in the plasma membrane of infected epithelial cells.
    Cell. 1980 May;20(1):45-54 PMID: 6248236
  43. Apical membrane aminopeptidase appears at site of cell-cell contact in cultured kidney epithelial cells.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):4132-6 PMID: 6933462
  44. Human lysosomes can be purified from diploid skin fibroblasts by free-flow electrophoresis.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):6139-43 PMID: 6934540
  45. Phase separation of integral membrane proteins in Triton X-114 solution.
    J Biol Chem. 1981 Feb 25;256(4):1604-7 PMID: 6257680
  46. Nucleotide sequences of the mRNA's encoding the vesicular stomatitis virus G and M proteins determined from cDNA clones containing the complete coding regions.
    J Virol. 1981 Aug;39(2):519-28 PMID: 6268840
  47. Structure of the influenza virus hemagglutinin.
    Curr Top Microbiol Immunol. 1981;94-95:1-74 PMID: 6171388
  48. Proteolytic activation of the influenza virus hemagglutinin: The structure of the cleavage site and the enzymes involved in cleavage.
    Virology. 1981 Dec;115(2):361-74 PMID: 7032055
  49. Infectious entry pathway of influenza virus in a canine kidney cell line.
    J Cell Biol. 1981 Dec;91(3 Pt 1):601-13 PMID: 7328111
  50. Sorting of sphingolipids in epithelial (Madin-Darby canine kidney) cells.
    J Cell Biol. 1987 Oct;105(4):1623-35 PMID: 3667693
  51. Galactosyltransferase and sialyltransferase are located in different subcellular compartments in HeLa cells.
    Exp Cell Res. 1987 Nov;173(1):267-73 PMID: 3119357
  52. Involvement of GTP-binding "G" proteins in transport through the Golgi stack.
    Cell. 1987 Dec 24;51(6):1053-62 PMID: 2826014
  53. Isolation of secretory vesicles from Saccharomyces cerevisiae.
    Anal Biochem. 1987 Nov 1;166(2):328-34 PMID: 3324821
  54. Isolation of a fraction enriched in the trans-Golgi network from baby hamster kidney cells.
    Exp Cell Res. 1988 Apr;175(2):248-65 PMID: 2834210
  55. Post-translational proteolysis in polypeptide hormone biosynthesis.
    Annu Rev Physiol. 1982;44:625-38 PMID: 7041809
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-12-20
Pages
4075-85
Language
English
Region
England
NLM ID
8208664
PMCID
PMC455116
Subset
IM
Grants
NIGMS NIH HHS · GM 11209-01 · United States
NIGMS NIH HHS · GM 11726-02 · United States
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2