Home LiteratureArticle Details
PMID: 6088557 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transcytosis of the G protein of vesicular stomatitis virus after implantation into the apical plasma membrane of Madin-Darby canine kidney cells. I. Involvement of endosomes and lysosomes.

The Journal of cell biology ·Vol. 99 ·No. 3 ·1984-09-00 ·Pages 796-82

Pesonen M, Ansorge W, Simons K

Abstract

The G protein of vesicular stomatitis virus, implanted into the apical plasma membrane of Madin-Darby canine kidney cells, is rapidly transcytosed to the basolateral membrane. In this and the accompanying paper (Pesonen, M., R. Bravo, and K. Simons, 1984, J. Cell Biol. 99:803-809.) we have studied the intracellular route by which the G protein traverses during transcytosis. Using Percoll density gradient centrifugation and free flow electrophoresis we could demonstrate that the G protein is endocytosed into a nonlysosomal compartment with a density of approximately 1.05 g/cm3, which has many of the characteristics of endosomes. Transcytosis to the basolateral membrane appeared to occur from this compartment. No direct evidence for the involvement of lysosomes in the transcytotic route could be obtained. No G protein was detected in the lysosomes when transcytosis of G protein was occurring. Moreover, at 21 degrees C when passage of G protein to the lysosomes was shown to be arrested, transcytosis of G protein could still be demonstrated.

MeSH Terms
Animals Biological Transport Cell Fractionation Cell Line Cell Membrane/metabolism Centrifugation, Density Gradient Dogs Kidney Kinetics Lysosomes/metabolism Membrane Glycoproteins Organoids/metabolism Thermodynamics Vesicular stomatitis Indiana virus/metabolism Viral Envelope Proteins Viral Proteins/metabolism
Chemicals
G protein, vesicular stomatitis virus Membrane Glycoproteins Viral Envelope Proteins Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pesonen M
Ansorge W
Simons K
References (43)
43 references, click to expand
  1. Evidence for a vesicular transport mechanism in hepatocytes for biliary secretion of immunoglobulin A.
    Science. 1980 Jun 13;208(4449):1276-8 PMID: 7375938
  2. Human lysosomes can be purified from diploid skin fibroblasts by free-flow electrophoresis.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):6139-43 PMID: 6934540
  3. Primary amines inhibit recycling of alpha 2M receptors in fibroblasts.
    Cell. 1980 May;20(1):37-43 PMID: 6156003
  4. Polarized distribution of viral envelope proteins in the plasma membrane of infected epithelial cells.
    Cell. 1980 May;20(1):45-54 PMID: 6248236
  5. Lysomotropic amines cause intracellular accumulation of receptors for epidermal growth factor.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3283-7 PMID: 6251452
  6. Fusion of Semliki forest virus with the plasma membrane can be induced by low pH.
    J Cell Biol. 1980 Oct;87(1):264-72 PMID: 7419594
  7. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  8. A cell line derived from normal dog kidney (MDCK) exhibiting qualities of papillary adenocarcinoma and of renal tubular epithelium.
    Cancer. 1970 Nov;26(5):1022-8 PMID: 4248968
  9. Intestinal transport of antibodies in the newborn rat.
    J Cell Biol. 1973 Jul;58(1):189-211 PMID: 4726306
  10. Mucosal and glandular distribution of immunoglobulin components: differential localization of free and bound SC in secretory epithelial cells.
    J Immunol. 1974 Apr;112(4):1553-9 PMID: 4205532
  11. Transepithelial transport in cell culture.
    Proc Natl Acad Sci U S A. 1976 Apr;73(4):1212-6 PMID: 1063404
  12. Polarized monolayers formed by epithelial cells on a permeable and translucent support.
    J Cell Biol. 1978 Jun;77(3):853-80 PMID: 567227
  13. Asymmetric budding of viruses in epithelial monlayers: a model system for study of epithelial polarity.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):5071-5 PMID: 283416
  14. Demonstration of protein asymmetries in the plasma membrane of cultured renal (MDCK) epithelial cells by lactoperoxidase-mediated iodination.
    FEBS Lett. 1979 Sep 15;105(2):201-4 PMID: 488350
  15. Chloroquine reduces the number of asialo-glycoprotein receptors in the hepatocyte plasma membrane.
    Biochem Pharmacol. 1979 Oct 1;28(19):2919-22 PMID: 518687
  16. Apical membrane aminopeptidase appears at site of cell-cell contact in cultured kidney epithelial cells.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):4132-6 PMID: 6933462
  17. Phase separation of integral membrane proteins in Triton X-114 solution.
    J Biol Chem. 1981 Feb 25;256(4):1604-7 PMID: 6257680
  18. Adsorptive endocytosis of Semliki Forest virus.
    J Mol Biol. 1980 Sep 25;142(3):439-54 PMID: 7463480
  19. Vesicular transport of cationized ferritin by the epithelium of the rat choroid plexus.
    J Cell Biol. 1981 Apr;89(1):131-9 PMID: 7228898
  20. Monensin interrupts the recycling of low density lipoprotein receptors in human fibroblasts.
    Cell. 1981 May;24(2):493-502 PMID: 6263497
  21. Galactose-specific recognition system of mammalian liver: receptor distribution on the hepatocyte cell surface.
    J Cell Biol. 1981 Sep;90(3):687-96 PMID: 6270158
  22. Evidence for the sorting of endocytic vesicle contents during the receptor-mediated transport of IgG across the newborn rat intestine.
    J Cell Biol. 1981 Oct;91(1):270-80 PMID: 7298722
  23. Mannose-specific endocytosis receptor of alveolar macrophages: demonstration of two functionally distinct intracellular pools of receptor and their roles in receptor recycling.
    J Cell Biol. 1982 Feb;92(2):417-24 PMID: 6277962
  24. Rapid acidification of endocytic vesicles containing alpha 2-macroglobulin.
    Cell. 1982 Mar;28(3):643-51 PMID: 6176331
  25. Recycling of the hepatocyte asialoglycoprotein receptor does not require delivery of ligand to lysosomes.
    J Biol Chem. 1982 Jun 10;257(11):6007-9 PMID: 6281252
  26. Pathway of vesicular stomatitis virus entry leading to infection.
    J Mol Biol. 1982 Apr 15;156(3):609-31 PMID: 6288961
  27. Monensin inhibits Semliki Forest virus penetration into culture cells.
    Proc Natl Acad Sci U S A. 1982 Sep;79(17):5297-301 PMID: 6957864
  28. Evidence that recycling of low density lipoprotein receptors does not depend on delivery of receptors to lysosomes.
    Biochem Biophys Res Commun. 1982 Aug 31;107(4):1551-8 PMID: 6291535
  29. Receptor-mediated endocytosis of transferrin and the uptake of fe in K562 cells: identification of a nonlysosomal acidic compartment.
    Proc Natl Acad Sci U S A. 1982 Oct;79(20):6186-90 PMID: 6292894
  30. Intracellular site of asialoglycoprotein receptor-ligand uncoupling: double-label immunoelectron microscopy during receptor-mediated endocytosis.
    Cell. 1983 Jan;32(1):277-87 PMID: 6130851
  31. Endocytosis and the recycling of plasma membrane.
    J Cell Biol. 1983 Jan;96(1):1-27 PMID: 6298247
  32. Penetration of Semliki Forest virus from acidic prelysosomal vacuoles.
    Cell. 1983 Mar;32(3):931-40 PMID: 6831562
  33. The role of intermediate vesicles in the adsorptive endocytosis and transport of ligand to lysosomes by human fibroblasts.
    J Cell Biol. 1983 Mar;96(3):644-50 PMID: 6220018
  34. Acidification of macrophage and fibroblast endocytic vesicles in vitro.
    Proc Natl Acad Sci U S A. 1983 Jun;80(11):3334-8 PMID: 6190176
  35. Reduced temperature prevents transfer of a membrane glycoprotein to the cell surface but does not prevent terminal glycosylation.
    Cell. 1983 Aug;34(1):233-43 PMID: 6883510
  36. Transepithelial transport of a viral membrane glycoprotein implanted into the apical plasma membrane of Madin-Darby canine kidney cells. I. Morphological evidence.
    J Cell Biol. 1983 Sep;97(3):627-37 PMID: 6885914
  37. Transepithelial transport of a viral membrane glycoprotein implanted into the apical plasma membrane of Madin-Darby canine kidney cells. II. Immunological quantitation.
    J Cell Biol. 1983 Sep;97(3):638-43 PMID: 6411736
  38. The importance of the endosome in intracellular traffic.
    Nature. 1983 Aug 25-31;304(5928):684-5 PMID: 6310401
  39. A monoclonal antibody against a 135-K Golgi membrane protein.
    EMBO J. 1982;1(12):1621-8 PMID: 7188254
  40. Viruses budding from either the apical or the basolateral plasma membrane domain of MDCK cells have unique phospholipid compositions.
    EMBO J. 1982;1(7):847-52 PMID: 6329709
  41. Transcytosis of the G protein of vesicular stomatitis virus after implantation into the apical membrane of Madin-Darby canine kidney cells. II. Involvement of the Golgi complex.
    J Cell Biol. 1984 Sep;99(3):803-9 PMID: 6088558
  42. Junctional complexes in various epithelia.
    J Cell Biol. 1963 May;17:375-412 PMID: 13944428
  43. Low temperature selectively inhibits fusion between pinocytic vesicles and lysosomes during heterophagy of 125I-asialofetuin by the perfused rat liver.
    J Biol Chem. 1980 Jun 25;255(12):5971-8 PMID: 6155379
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.99.3.796
Published
1984-09-00
Pages
796-82
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2113380
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.99.3.796 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2