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PMID: 2139695 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Activated human T lymphocytes express MHC class I heavy chains not associated with beta 2-microglobulin.

The Journal of experimental medicine ·Vol. 171 ·No. 5 ·1990-05-01 ·Pages 1431-42

Schnabl E, Stockinger H, Majdic O, Gaugitsch H, Lindley IJ, Maurer D, Hajek-Rosenmayr A, Knapp W

Abstract

We present here the molecular characterization of a new activation-induced surface structure on human T lymphocytes, termed LA45, with high homology (93% at protein level) to MHC class I molecules. Antigen modulation and sequential immunoprecipitation experiments revealed that LA45 and HLA class I proteins do not crossreact with the corresponding antibodies. Furthermore, LA45 is not associated with beta 2-m. On the other hand, we could show that the separation of HLA-A,B,C and beta 2m molecules, induced by SDS-denaturation, leads to a conformational change in the heavy chain in such a way that it becomes reactive with LA45. The 90/45 kD LA45 proteins thus appear to be non-beta 2m-associated MHC class I alpha chains that are selectively expressed by activated but not by resting human T lymphocytes.

MeSH Terms
Amino Acid Sequence Antibodies, Monoclonal Antigens, CD/analysis Antigens, Differentiation, T-Lymphocyte/analysis Base Sequence Blotting, Western CD3 Complex Cells, Cultured Cloning, Molecular Histocompatibility Antigens Class I/analysis,genetics Humans Lymphocyte Activation Macromolecular Substances Molecular Sequence Data Receptors, Antigen, T-Cell/analysis Sequence Homology, Nucleic Acid T-Lymphocytes/immunology beta 2-Microglobulin/analysis
Chemicals
Antibodies, Monoclonal Antigens, CD Antigens, Differentiation, T-Lymphocyte CD3 Complex Histocompatibility Antigens Class I LA45 antigen Macromolecular Substances Receptors, Antigen, T-Cell beta 2-Microglobulin
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Schnabl E
Institute of Immunology, Vienna, Austria.
Stockinger H
Majdic O
Gaugitsch H
Lindley I J
Maurer D
Hajek-Rosenmayr A
Knapp W
References (30)
30 references, click to expand
  1. Beta 2-microglobulin is not required for cell surface expression of the murine class I histocompatibility antigen H-2Db or of a truncated H-2Db.
    Proc Natl Acad Sci U S A. 1986 Oct;83(19):7447-51 PMID: 3532114
  2. Monoclonal antibodies raised against denatured HLA-B locus heavy chains permit biochemical characterization of certain HLA-C locus products.
    J Immunol. 1986 Oct 1;137(7):2299-306 PMID: 3760563
  3. Isolation of a human major histocompatibility complex class I gene encoding a nonubiquitous molecule expressed on activated lymphocytes.
    Proc Natl Acad Sci U S A. 1987 May;84(9):2872-6 PMID: 3033667
  4. Structure, expression, and molecular mapping of a divergent member of the class I HLA gene family.
    Proc Natl Acad Sci U S A. 1987 Jun;84(12):4224-8 PMID: 2438694
  5. Structure of the human class I histocompatibility antigen, HLA-A2.
    Nature. 1987 Oct 8-14;329(6139):506-12 PMID: 3309677
  6. Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system.
    Proc Natl Acad Sci U S A. 1987 Dec;84(23):8573-7 PMID: 2825196
  7. Isolation and nucleotide sequence of a cDNA clone encoding a novel HLA class I gene.
    J Immunol. 1988 Jun 1;140(11):4024-30 PMID: 3131426
  8. Nature of polymorphism in HLA-A, -B, and -C molecules.
    Proc Natl Acad Sci U S A. 1988 Jun;85(11):4005-9 PMID: 3375250
  9. HLA-E. A novel HLA class I gene expressed in resting T lymphocytes.
    J Immunol. 1988 Aug 1;141(3):897-904 PMID: 3260916
  10. Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties.
    Nucleic Acids Res. 1988 Aug 11;16(15):7583-600 PMID: 2970625
  11. Diversity and diversification of HLA-A,B,C alleles.
    J Immunol. 1989 Jun 1;142(11):3937-50 PMID: 2715640
  12. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  13. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  14. Use of a monoclonal antibody (W6/32) in structural studies of HLA-A,B,C, antigens.
    J Immunol. 1979 Jul;123(1):342-9 PMID: 87477
  15. Heavy chain of HLA-A and HLA-B antigens is conformationally labile: a possible role for beta 2-microglobulin.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3844-8 PMID: 91170
  16. Assembly and maturation of HLA-A and HLA-B antigens in vivo.
    Cell. 1979 Dec;18(4):979-91 PMID: 93026
  17. Major histocompatibility antigens: the human (HLA-A, -B, -C) and murine (H-2K, H-2D) class I molecules.
    Cell. 1981 May;24(2):287-99 PMID: 7016338
  18. Alloantibodies to PHA-activated lymphocytes detect human Qa-like antigens.
    J Immunol. 1984 Jan;132(1):165-9 PMID: 6606664
  19. A simple and very efficient method for generating cDNA libraries.
    Gene. 1983 Nov;25(2-3):263-9 PMID: 6198242
  20. Interaction between major histocompatibility complex antigens and epidermal growth factor receptors on human cells.
    J Cell Biol. 1984 Feb;98(2):725-31 PMID: 6319431
  21. Beta 2-microglobulin from serum associates with MHC class I antigens on the surface of cultured cells.
    Nature. 1984 Apr 12-18;308(5960):642-5 PMID: 6369147
  22. Low and high affinity cellular receptors for interleukin 2. Implications for the level of Tac antigen.
    J Exp Med. 1984 Oct 1;160(4):1126-46 PMID: 6090574
  23. Rapid and sensitive protein similarity searches.
    Science. 1985 Mar 22;227(4693):1435-41 PMID: 2983426
  24. New human MHC class I antigens segregating with HLA-A antigens detected on some lymphocyte subpopulations.
    Hum Immunol. 1986 Feb;15(2):175-97 PMID: 3485086
  25. Recombinant interferon-gamma induces interleukin 2 receptors on human peripheral blood monocytes.
    J Immunol. 1986 Mar 15;136(6):2171-5 PMID: 3081639
  26. Slower processing, weaker beta 2-M association, and lower surface expression of H-2Ld are influenced by its amino terminus.
    J Immunol. 1986 Aug 1;137(3):916-23 PMID: 3522739
  27. The major histocompatibility complex class I heavy chain as a structural subunit of the human cell membrane insulin receptor: implications for the range of biological functions of histocompatibility antigens.
    Proc Natl Acad Sci U S A. 1986 Aug;83(16):6007-11 PMID: 3090548
  28. New class I in man: serological and molecular characterization.
    Hum Immunol. 1986 Sep;17(1):3-20 PMID: 3488984
  29. Cross-linking of insulin receptors to MHC antigens in human B lymphocytes: evidence for selective molecular interactions.
    J Immunol. 1986 Oct 1;137(7):2293-8 PMID: 3531328
  30. Efficient cell surface expression of class II MHC molecules in the absence of associated invariant chain.
    J Exp Med. 1986 Nov 1;164(5):1478-89 PMID: 3464691
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.171.5.1431
Published
1990-05-01
Pages
1431-42
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2187879
Subset
IM
Databases
GENBANK
X51745
External Links
PubMed source DOI: 10.1084/jem.171.5.1431 PMC Full Text View journal details
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