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PMID: 2142530 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

nit-2, the major positive-acting nitrogen regulatory gene of Neurospora crassa, encodes a sequence-specific DNA-binding protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 14 ·1990-07-00 ·Pages 5331-5

Fu YH, Marzluf GA

Abstract

The nit-2 major nitrogen regulatory gene of Neurospora crassa turns on the expression of various unlinked structural genes that specify nitrogen-catabolic enzymes under nitrogen-limitation conditions. The nit-2 gene encodes a protein of 1036 amino acid residues with a single zinc finger and a downstream basic region that may make up a DNA-binding domain. The zinc-finger domain of the NIT2 protein was synthesized in two ways to examine its DNA-binding activity with gel-band-mobility shift and DNA-footprint experiments. The NIT2 protein binds to specific DNA recognition elements that are located upstream of nitrogen-regulated structural genes. Each recognition element contains at least two copies of a core sequence whose consensus is TATCTA.

MeSH Terms
Base Sequence Binding Sites Cloning, Molecular DNA-Binding Proteins/biosynthesis,genetics,isolation & purification Escherichia coli/genetics Gene Expression Genes, Bacterial Genes, Fungal Metalloproteins/genetics Molecular Sequence Data Neurospora/genetics Neurospora crassa/genetics,metabolism Nitrogen Fixation/genetics Promoter Regions, Genetic Recombinant Fusion Proteins/biosynthesis,isolation & purification Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
DNA-Binding Proteins Metalloproteins Recombinant Fusion Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fu Y H
Department of Biochemistry, Ohio State University, Columbus 43210.
Marzluf G A
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.14.5331
Published
1990-07-00
Pages
5331-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54317
Subset
IM
Grants
NIGMS NIH HHS · GM-23367 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.14.5331 PMC Full Text View journal details
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