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PMID: 2143774 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

T cell receptor-mediated negative selection of autoreactive T lymphocyte precursors occurs after commitment to the CD4 or CD8 lineages.

The Journal of experimental medicine ·Vol. 172 ·No. 3 ·1990-09-01 ·Pages 835-45

Guidos CJ, Danska JS, Fathman CG, Weissman IL

Abstract

To identify the maturational stage(s) during which T cell receptor (TCR)-mediated positive and negative selection occurs, we followed the development of CD4+8- and CD4-8+ T cells from TCRlo CD4+8+ thymic blasts in the presence of different positive and negative selecting (major histocompatibility complex or Mls) elements. We describe novel lineage-committed transitional intermediates that are TCRmed CD4+8lo or TCRmed CD4lo8+, and that show evidence of having been positively selected. Furthermore, negative selection is not evident until after cells have attained one of the TCRmed transitional phenotypes. Accordingly, we propose that negative selection in normal mice occurs only after TCRlo CD4+8+ precursors have been positively selected into either the CD4 or CD8 lineage.

MeSH Terms
Animals Antigens, Differentiation, T-Lymphocyte/immunology,isolation & purification CD3 Complex CD4 Antigens/immunology CD8 Antigens Crosses, Genetic Mice Mice, Inbred Strains Models, Biological Oligonucleotide Probes Polymerase Chain Reaction Receptors, Antigen, T-Cell/genetics,immunology,isolation & purification Species Specificity T-Lymphocytes/cytology,immunology Thymus Gland/immunology Transcription, Genetic
Chemicals
Antigens, Differentiation, T-Lymphocyte CD3 Complex CD4 Antigens CD8 Antigens Oligonucleotide Probes Receptors, Antigen, T-Cell
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Guidos C J
Department of Pathology, Stanford University School of Medicine, California 94305.
Danska J S
Fathman C G
Weissman I L
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.172.3.835
Published
1990-09-01
Pages
835-45
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188558
Subset
IM
Grants
NIAID NIH HHS · AI-0720 · United States
NIAID NIH HHS · AI-27989 · United States
External Links
PubMed source DOI: 10.1084/jem.172.3.835 PMC Full Text View journal details
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