Home LiteratureArticle Details
PMID: 2143982 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Lateral mobility of the phospholipase C-activating vasopressin V1-type receptor in A7r5 smooth muscle cells: a comparison with the adenylate cyclase-coupled V2-receptor.

The EMBO journal ·Vol. 9 ·No. 9 ·1990-09-00 ·Pages 2693-9

Jans DA, Peters R, Fahrenholz F

Abstract

The present work examines lateral mobility of the vasopressin V1-type receptor, representing the first determination of lateral mobility of a hormone receptor coupled to phospholipase C activation. The V1-receptor of A7r5 smooth muscle cells was characterized for [Arg8] vasopressin (AVP) binding properties and affinity for the fluorescent vasopressin analogue 1-deamino[8-lysine (N6-tetramethylrhodamylaminothiocarbonyl)] vasopressin (TR-LVP). TR-LVP was biologically active in A7r5 cells, inducing inositol 1,4,5-trisphosphate turnover in similar fashion to AVP. TR-LVP was used to specifically label the V1-receptor of living A7r5 cells, and lateral mobility of the V1-receptor was measured using the technique of fluorescence microphotolysis. The apparent lateral diffusion coefficient (D) at 37 degrees C was 5.1 x 10(-10) cm2/s, falling to 2.9 x 10(-10) cm2/s at 13 degrees C. These D values are higher than comparable values for the adenylate cyclase-activating vasopressin V2-receptor of LLC-PK1 renal epithelial cells analysed with the same fluorescent ligand. In contrast to the V2-receptor, no marked temperature dependence was observed for the V1-receptor mobile fraction (f). From 37 degrees C to 13 degrees C, f was relatively low (between 0.4 and 0.5) consistent with V1-receptor immobilization through internalization, which is rapid even at room temperature in A7r5 cells. These differences between V1- and V2-receptor lateral mobility are discussed in terms of the implications for their respective signal transduction systems.

MeSH Terms
Adenylyl Cyclases/metabolism Animals Arginine Vasopressin/metabolism Cell Line Cell Membrane/metabolism Enzyme Activation Kinetics Muscle, Smooth Receptors, Angiotensin/metabolism Receptors, Vasopressin Spectrometry, Fluorescence Thermodynamics Type C Phospholipases/metabolism
Chemicals
Receptors, Angiotensin Receptors, Vasopressin Arginine Vasopressin Type C Phospholipases Adenylyl Cyclases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jans D A
Max-Planck-Institut für Biophsik, Frankfurt am Main, FRG.
Peters R
Fahrenholz F
References (45)
45 references, click to expand
  1. Membrane receptors.
    Annu Rev Biochem. 1974;43(0):169-214 PMID: 4368906
  2. Dependence of urokinase-type-plasminogen-activator induction on cyclic AMP-dependent protein kinase activation in LLC-PK1 cells.
    Biochem J. 1987 Apr 15;243(2):413-8 PMID: 2820380
  3. Characterization of two putative smooth muscle cell lines from rat thoracic aorta.
    Exp Cell Res. 1976 Mar 15;98(2):349-66 PMID: 943301
  4. Site-site interactions among insulin receptors. Characterization of the negative cooperativity.
    J Biol Chem. 1976 Apr 10;251(7):1877-88 PMID: 5434
  5. Membrane receptors for hormones and neurotransmitters.
    J Cell Biol. 1976 Aug;70(2 pt 1):261-86 PMID: 7569
  6. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  7. Mobility measurement by analysis of fluorescence photobleaching recovery kinetics.
    Biophys J. 1976 Sep;16(9):1055-69 PMID: 786399
  8. Liposomes from ionic, single-chain amphiphiles.
    Biochemistry. 1978 Sep 5;17(18):3759-68 PMID: 698196
  9. Quantitative determination of the lateral diffusion coefficients of the hormone-receptor complexes of insulin and epidermal growth factor on the plasma membrane of cultured fibroblasts.
    Proc Natl Acad Sci U S A. 1978 Nov;75(11):5353-7 PMID: 214784
  10. A variant of S49 mouse lymphoma cells with enhanced secretion of cyclic AMP.
    J Cell Physiol. 1979 Sep;100(3):579-88 PMID: 226556
  11. Removal of the surface-bound human choriogonadotropin results in the cessation of hormonal responses in cultured Leydig tumor cells.
    J Biol Chem. 1981 Nov 25;256(22):11420-3 PMID: 6271747
  12. Rotational diffusion of epidermal growth factor complexed to cell surface receptors reflects rapid microaggregation and endocytosis of occupied receptors.
    Proc Natl Acad Sci U S A. 1981 Nov;78(11):6981-5 PMID: 6273899
  13. Lateral diffusion of epidermal growth factor complexed to its surface receptors does not account for the thermal sensitivity of patch formation and endocytosis.
    Biochemistry. 1982 Mar 30;21(7):1667-72 PMID: 6282315
  14. Theoretical analysis of fluorescence photobleaching recovery experiments.
    Biophys J. 1983 Jan;41(1):95-7 PMID: 6824758
  15. Interactions of vasopressin agonists and antagonists with membrane receptors.
    Eur J Pharmacol. 1984 Apr 13;100(1):47-58 PMID: 6327326
  16. High affinity epidermal growth factor receptors on the surface of A431 cells have restricted lateral diffusion.
    EMBO J. 1984 Aug;3(8):1843-7 PMID: 6090128
  17. Inositol trisphosphate, a novel second messenger in cellular signal transduction.
    Nature. 1984 Nov 22-28;312(5992):315-21 PMID: 6095092
  18. Desensitization of LLC-PK1 cells by vasopressin results in receptor down-regulation.
    Mol Cell Endocrinol. 1985 May;40(2-3):193-204 PMID: 2989032
  19. A smooth muscle cell line suitable for the study of voltage sensitive calcium channels.
    Biochem Biophys Res Commun. 1985 Jul 16;130(1):447-53 PMID: 2411261
  20. Vasopressin induced production of inositol trisphosphate and calcium efflux in a smooth muscle cell line.
    Biochem Biophys Res Commun. 1985 Aug 30;131(1):469-76 PMID: 2994663
  21. Internalization, recycling, and redistribution of vasopressin receptors in rat hepatocytes.
    J Biol Chem. 1985 Oct 15;260(23):12641-6 PMID: 2995382
  22. Photoaffinity labelling of the renal V2 vasopressin receptor. Identification and enrichment of a vasopressin-binding subunit.
    Eur J Biochem. 1985 Nov 4;152(3):589-95 PMID: 2996895
  23. Activation of V1-receptors by vasopressin stimulates inositol phospholipid hydrolysis and arachidonate metabolism in human platelets.
    Biochem J. 1986 Jan 1;233(1):83-91 PMID: 3006664
  24. Vasopressin receptor-adenylate cyclase interactions. Studies in an intact cultured renal epithelial cell line (LLC-PK1).
    Miner Electrolyte Metab. 1986;12(1):64-70 PMID: 3007962
  25. Alpha-subunits of Ns are released from the plasma membrane following cholera toxin activation.
    FEBS Lett. 1986 May 12;200(2):333-6 PMID: 3086121
  26. Isolation of a mutant LLC-PK1 cell line defective in hormonal responsiveness. A pleiotropic lesion in receptor function.
    Eur J Biochem. 1986 Oct 15;160(2):407-12 PMID: 3021458
  27. Solubilization of the vasopressin receptor from rat liver plasma membranes. Evidence for a receptor X GTP-binding protein complex.
    J Biol Chem. 1986 Dec 25;261(36):16871-7 PMID: 2946691
  28. Fluorescence microphotolysis to measure nucleocytoplasmic transport and intracellular mobility.
    Biochim Biophys Acta. 1986 Dec 22;864(3-4):305-59 PMID: 3539193
  29. The labelling of polyphosphoinositides with [32P]Pi and the accumulation of inositol phosphates in vasopressin-stimulated hepatocytes.
    Biochem J. 1986 Sep 1;238(2):491-9 PMID: 3026353
  30. Guanine nucleotide regulation of [3H]vasopressin binding to liver plasma membranes and solubilized receptors. Evidence for the involvement of a guanine nucleotide regulatory protein.
    Biochem J. 1986 Dec 1;240(2):361-5 PMID: 2949738
  31. G proteins: a family of signal transducers.
    Annu Rev Cell Biol. 1986;2:391-419 PMID: 3103658
  32. G proteins: transducers of receptor-generated signals.
    Annu Rev Biochem. 1987;56:615-49 PMID: 3113327
  33. Long-term stimulation of cAMP production in LLC-PK1 pig kidney epithelial cells by salmon calcitonin or a photoactivatable analogue of vasopressin.
    Biochim Biophys Acta. 1987 Oct 1;930(3):392-400 PMID: 2820505
  34. Purification and characterization of the rat liver vasopressin (V1) receptor.
    J Biol Chem. 1987 Oct 15;262(29):14049-55 PMID: 2958456
  35. G protein involvement in receptor-effector coupling.
    J Biol Chem. 1988 Feb 25;263(6):2577-80 PMID: 2830256
  36. GTP analogues cause release of the alpha subunit of the GTP binding protein, GO, from the plasma membrane of NG108-15 cells.
    Biochem Biophys Res Commun. 1988 Apr 15;152(1):243-51 PMID: 3128978
  37. The epidermal growth factor receptor as a multifunctional allosteric protein.
    Biochemistry. 1988 May 3;27(9):3119-23 PMID: 3291943
  38. Lateral mobility of proteins and lipids in the red cell membrane and the activation of adenylate cyclase by beta-adrenergic receptors.
    FEBS Lett. 1988 Jul 4;234(1):1-7 PMID: 2839357
  39. Properties of the insulin receptor ectodomain.
    Proc Natl Acad Sci U S A. 1988 Oct;85(20):7516-20 PMID: 3050997
  40. Subunit dissociation is the mechanism for hormonal activation of the Gs protein in native membranes.
    J Biol Chem. 1988 Nov 25;263(33):17239-42 PMID: 3141418
  41. Down-regulation of calcitonin receptors in T47D cells by internalization of calcitonin-receptor complexes.
    Mol Cell Endocrinol. 1988 Jul;58(1):9-15 PMID: 2850244
  42. Signal transduction by allosteric receptor oligomerization.
    Trends Biochem Sci. 1988 Nov;13(11):443-7 PMID: 3075366
  43. Stimulation of beta-adrenergic receptors of S49 lymphoma cells redistributes the alpha subunit of the stimulatory G protein between cytosol and membranes.
    Proc Natl Acad Sci U S A. 1989 Oct;86(20):7900-3 PMID: 2554294
  44. The adenylate cyclase-coupled vasopressin V2-receptor is highly laterally mobile in membranes of LLC-PK1 renal epithelial cells at physiological temperature.
    EMBO J. 1989 Sep;8(9):2481-8 PMID: 2531084
  45. A microfluorimetric study of translational diffusion in erythrocyte membranes.
    Biochim Biophys Acta. 1974 Nov 15;367(3):282-94 PMID: 4429678
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-09-00
Pages
2693-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC551974
Subset
IM
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2