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PMID: 2144000 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The alpha 1-alpha 6 subunits of integrins are characteristically expressed in distinct segments of developing and adult human nephron.

The Journal of cell biology ·Vol. 111 ·No. 3 ·1990-09-00 ·Pages 1245-54

Korhonen M, Ylänne J, Laitinen L, Virtanen I

Abstract

We studied the distribution of the alpha 1-alpha 6 subunits of beta 1 integrins in developing and adult human kidney using a panel of mAbs in indirect immunofluorescence microscopy. Uninduced mesenchyme displayed a diffuse immunoreactivity for only the alpha 1 integrin subunit. At the S-shaped body stage of nephron development, several of the alpha subunits were characteristically expressed in distinct fetal nephron segments, and the pattern was retained also in the adult nephron. Thus, the alpha 1 subunit was characteristically expressed in mesangial and endothelial cells, the alpha 2 in glomerular endothelium and distal tubules, the alpha 3 in podocytes, Bowman's capsule, and distal tubules, and the alpha 6 subunit basally in all tubules, and only transiently in podocytes during development. Unlike the alpha 3 and alpha 6 subunits, the alpha 2 subunit displayed an overall cell surface distribution in distal tubules. It was also distinctly expressed in glomerular endothelia during glomerulogenesis. The beta 4 subunit was expressed only in fetal collecting ducts, and hence the alpha 6 subunit seems to be complexed with the beta 1 rather than beta 4 subunit in human kidney. Of the two fibronectin receptor alpha subunits, alpha 4 and alpha 5, only the latter was expressed, confined to endothelia of developing and adult blood vessels, suggesting that these receptor complexes play a minor role during nephrogenesis. The present results suggest that distinct integrins play a role during differentiation of specific nephron segments. They also indicate that alpha 3 beta 1 and alpha 6 beta 1 integrin complexes may function as basement membrane receptors in podocytes and tubular epithelial cells.

MeSH Terms
Adult Antibodies, Monoclonal Basement Membrane/metabolism Endothelium/cytology Fetus/metabolism Fibronectins/metabolism Fluorescent Antibody Technique Gene Expression Humans Integrins/biosynthesis,physiology Kidney Glomerulus/cytology Kidney Tubules, Distal/cytology Nephrons/embryology,growth & development,metabolism Receptors, Fibronectin Receptors, Immunologic/physiology
Chemicals
Antibodies, Monoclonal Fibronectins Integrins Receptors, Fibronectin Receptors, Immunologic
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Korhonen M
Department of Anatomy, University of Helsinki, Finland.
Ylänne J
Laitinen L
Virtanen I
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.111.3.1245
Published
1990-09-00
Pages
1245-54
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2116295
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.111.3.1245 PMC Full Text View journal details
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