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PMID: 21505417 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Crystal structure of the intraflagellar transport complex 25/27.

The EMBO journal ·Vol. 30 ·No. 10 ·2011-05-18 ·Pages 1907-18

Bhogaraju S, Taschner M, Morawetz M, Basquin C, Lorentzen E

Abstract

The cilium is an important organelle that is found on many eukaryotic cells, where it serves essential functions in motility, sensory reception and signalling. Intraflagellar transport (IFT) is a vital process for the formation and maintenance of cilia. We have determined the crystal structure of Chlamydomonas reinhardtii IFT25/27, an IFT sub-complex, at 2.6 Å resolution. IFT25 and IFT27 interact via a conserved interface that we verify biochemically using structure-guided mutagenesis. IFT27 displays the fold of Rab-like small guanosine triphosphate hydrolases (GTPases), binds GTP and GDP with micromolar affinity and has very low intrinsic GTPase activity, suggesting that it likely requires a GTPase-activating protein (GAP) for robust GTP turnover. A patch of conserved surface residues contributed by both IFT25 and IFT27 is found adjacent to the GTP-binding site and could mediate the binding to other IFT proteins as well as to a potential GAP. These results provide the first step towards a high-resolution structural understanding of the IFT complex.

MeSH Terms
Amino Acid Sequence Carrier Proteins/chemistry,genetics,metabolism Chlamydomonas reinhardtii/chemistry,genetics Crystallography, X-Ray DNA Mutational Analysis Guanosine Diphosphate/metabolism Guanosine Triphosphate/metabolism Models, Molecular Molecular Sequence Data Mutagenesis, Site-Directed Protein Binding Protein Interaction Domains and Motifs Protein Interaction Mapping Protein Structure, Quaternary Sequence Homology, Amino Acid
Chemicals
Carrier Proteins Guanosine Diphosphate Guanosine Triphosphate
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bhogaraju Sagar
Department of Structural Cell Biology, Max-Planck-Institute of Biochemistry, Martinsried, Germany.
Taschner Michael
Morawetz Michaela
Basquin Claire
Lorentzen Esben
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
1460-2075
DOI
10.1038/emboj.2011.110
Published
2011-05-18
Epub
2011-00-19
Pages
1907-18
Language
English
Region
England
NLM ID
8208664
PMCID
PMC3098482
Subset
IM
Databases
PDB
2YC2, 2YC4
External Links
PubMed source DOI: 10.1038/emboj.2011.110 PMC Full Text View journal details
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